IPREM/LCABIE Laboratoire de Chimie Analytique Bio-inorganique et Environnement

Background Information
Chromatin immunoprecipitaton (ChIP) provides an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions could possibly be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could possibly be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Package deal is an entire set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle may be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

• Terribly fast course of: your whole course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
• Helpful and versatile: the bundle is suitable for getting ready every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
• Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

Principle & Course of
The ChromaFlash Chromatin Extraction Package deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, modern and frozen tissues, and so forth. The amount of cells and tissues for each preparation could possibly be 1 x 10⁵ to 5 x 10⁶ cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 10⁶ cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 10⁶  cells or per 50 mg tissues.

Background Information
Chromatin immunoprecipitation (ChIP) provides an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions could possibly be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could possibly be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.

miRNA Extraction Kit
K1456-50	Biovision	50 Preps	EUR 661.2

ExoDNA? Extraction Kit
K1242-25	Biovision	each	EUR 705.6

ExoDNA? Extraction Kit
K1242-50	Biovision	each	EUR 1057.2

Total Protein Extraction Kit
BSP003	Bio Basic	50Preps	EUR 130.99

Plant Protein Extraction Kit
BSP004	Bio Basic	20Preps	EUR 133.08

Nuclear Protein Extraction Kit
BSP009	Bio Basic	50Preps	EUR 106.98

FFPE Protein Extraction Kit
BSP058	Bio Basic	50Preps	EUR 122.64

Membrane Protein Extraction Kit
55R-1357	Fitzgerald	50 assays	EUR 888
Description: Membrane Protein Extraction Kit for use in the research laboratory

Mammalian Cell Extraction Kit
55R-1358	Fitzgerald	500 assays	EUR 457.2
Description: Mammalian Cell Extraction Kit for use in the research laboratory

Quick Gel Extraction Kit
20-abx098085	Abbexa	EUR 343.20 EUR 243.60	200 rxns 50 rxns

Up RNA Extraction Kit
20-abx098087	Abbexa	EUR 309.60 EUR 126.00	100 ml 3 ml

Plant RNA extraction Kit
20-abx098088	Abbexa	EUR 309.60 EUR 126.00	100 ml 3 ml

Total Protein Extraction Kit
K3011010	Biochain	1 kit	EUR 444
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein Extraction Kit
K2113-50	ApexBio	50 assays	EUR 652.8

Mammalian Cell Extraction Kit
K2114-500	ApexBio	500 assays	EUR 385.2

Bacterial RNA Extraction Kit
R403-01	Vazyme	100 rxn	EUR 186

EpiQuik Nuclear Extraction Kit
OP-0002-1	EpiGentek	100 Extractions	EUR 211.2

EpiQuik Nuclear Extraction Kit
OP-0002	EpiGentek	EUR 350.58 EUR 211.20	100 Assays 100 Extractions

Membrane Protein Extraction Kit
MPE01-12S	ProFoldin	0.5 ml	EUR 117.02
Description: This product includes 0.5 ml of 12 solutions for screening and micro-scale preparative extraction of membrane proteins (up to 1.5 mg) from the cell membranes.

Membrane Protein Extraction Kit
K268-50	Biovision	each	EUR 646.8

Mammalian Cell Extraction Kit
K269-500	Biovision	each	EUR 352.8

Plant Tissue Extraction Kit
K296-50	Biovision	each	EUR 548.4

Frit Kit
FRIT-KIT	Next Advance	1each	EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit
PACK-KIT	Next Advance	1pack	EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

EpiQuik Chromatin Accessibility Assay Kit
P-1047-48	EpiGentek	48 Reactions	EUR 611.6

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
P-2002-1	EpiGentek	24 Reactions	EUR 312.4

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
P-2002-2	EpiGentek	48 Reactions	EUR 500.5

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
P-2002-3	EpiGentek	96 Reactions	EUR 738.1

ChromaFlash Chromatin Isolation & Shearing Kit
P-2023-100	EpiGentek	100 Preparations	EUR 189.2

EpiQuik Chromatin Accessibility Assay Kit
P-1047	EpiGentek	EUR 910.86 EUR 611.60	48 Assays 48 Reactions

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
P-2002	EpiGentek	EUR 1084.86 EUR 312.40 EUR 500.50 EUR 738.10	96 Reactions 24 Reactions 48 Reactions 96 Reactions

ChromaFlash Chromatin Isolation & Shearing Kit
P-2023	EpiGentek	EUR 324.48 EUR 189.20	100 Preperations 100 Preparations

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 319.2

Triton Extraction Buffer(TEB), for Histon Extraction
T8300-100	GenDepot	100ml	EUR 151.2

Triton Extraction Buffer(TEB), for Histon Extraction
T8300-500	GenDepot	500ml	EUR 170.4

DNA Extraction Kit in Tubes
D100T	Cygnus Technologies	1 kit (50 tubes)	EUR 562.8
Description: DNA Extraction Kit in Tubes by Cygnus Technologies is available in Europe via Gentaur.

DNA Extraction Kit in Wells
D100W	Cygnus Technologies	1 kit (96 wells plate)	EUR 562.8
Description: DNA Extraction Kit in Wells by Cygnus Technologies is available in Europe via Gentaur.

Inclusion Body Protein Extraction Kit
BS686	Bio Basic	1kit, 10prep	EUR 127.86

Mammalian Total Protein Extraction Kit
20-abx098853	Abbexa	EUR 427.20 EUR 126.00	100 ml 2 ml

Mammalian Membrane Protein Extraction Kit
20-abx098855	Abbexa	EUR 126.00 EUR 460.80	2 rxns 50 rxns

Up Plus RNA Extraction Kit
20-abx098093	Abbexa	EUR 444.00 EUR 126.00	100 rxns 2 rxns