IPREM/LCABIE Laboratoire de Chimie Analytique Bio-inorganique et Environnement

Background Information
Chromatin immunoprecipitaton (ChIP) provides an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions could possibly be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could possibly be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Package deal is an entire set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle may be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

• Terribly fast course of: your whole course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
• Helpful and versatile: the bundle is suitable for getting ready every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
• Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

Principle & Course of
The ChromaFlash Chromatin Extraction Package deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, modern and frozen tissues, and so forth. The amount of cells and tissues for each preparation could possibly be 1 x 10⁵ to 5 x 10⁶ cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 10⁶ cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 10⁶  cells or per 50 mg tissues.

Background Information
Chromatin immunoprecipitation (ChIP) provides an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions could possibly be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could possibly be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.

miRNA Extraction Kit
K1456-50	Biovision	50 Preps	EUR 661.2

ExoDNA? Extraction Kit
K1242-25	Biovision	each	EUR 705.6

ExoDNA? Extraction Kit
K1242-50	Biovision	each	EUR 1057.2

Up RNA Extraction Kit
20-abx098087	Abbexa	EUR 309.60 EUR 126.00	100 ml 3 ml

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
P-2002	EpiGentek	EUR 1084.86 EUR 312.40 EUR 500.50 EUR 738.10	96 Reactions 24 Reactions 48 Reactions 96 Reactions

Quick Gel Extraction Kit
20-abx098085	Abbexa	EUR 343.20 EUR 243.60	200 rxns 50 rxns

Plant RNA extraction Kit
20-abx098088	Abbexa	EUR 309.60 EUR 126.00	100 ml 3 ml

Chromatin protein Cren7 Antibody (HRP)
20-abx109204	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Gel Extraction Kit (100 preps)
9K-006-0001	Bio Basic	100preps	EUR 283.42

Gel Extraction Kit (200 preps)
9K-006-0002	Bio Basic	200preps, 200prep	EUR 418.09

Chromatin protein Cren7 Antibody (FITC)
20-abx107787	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

FFPE Protein Extraction Kit
BSP058	Bio Basic	50Preps	EUR 122.64

Exosomal RNA Extraction Kit
P200R,-part-of-P200	101Bio	-	Ask for price

Exosomal DNA Extraction Kit
P230-25	101Bio	-	Ask for price

Exosomal DNA Extraction Kit
P230-50	101Bio	-	Ask for price

Plant Tissue Extraction Kit
K296-50	Biovision	each	EUR 548.4

Chromatin Modifying Protein 2A Protein
20-abx261989	Abbexa	EUR 5388.00 EUR 393.60 EUR 276.00	1 mg 20 ug 5 ug

Chromatin Modifying Protein 2B Protein
20-abx262025	Abbexa	EUR 5388.00 EUR 393.60 EUR 276.00	1 mg 20 ug 5 ug

Chromatin Modifying Protein 4A Protein
20-abx263203	Abbexa	EUR 276.00 EUR 1779.60 EUR 393.60	1 µg 50 ug 5 ug

Chromatin Modifying Protein 1A Protein
20-abx263326	Abbexa	EUR 1930.80 EUR 393.60 EUR 276.00	100 ug 10 ug 2 µg

Chromatin Protein Cren7 (creN7) Antibody
20-abx109437	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

ATRX, Chromatin Remodeler (ATRX) Antibody
20-abx325123	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

ATRX, Chromatin Remodeler (ATRX) Antibody
abx331300-100ul	Abbexa	100 ul	EUR 510

Total Protein Extraction Kit
BSP003	Bio Basic	50Preps	EUR 130.99

Plant Protein Extraction Kit
BSP004	Bio Basic	20Preps	EUR 133.08

Total Protein Extraction Kit
K3011010	Biochain	1 kit	EUR 367

Bacterial RNA Extraction Kit
R403-01	Vazyme	100 rxn	EUR 186

Up Plus RNA Extraction Kit
20-abx098093	Abbexa	EUR 444.00 EUR 126.00	100 rxns 2 rxns

Chromatin protein Cren7 Antibody (Biotin)
20-abx106375	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Mammalian Cell Extraction Kit
55R-1358	Fitzgerald	500 assays	EUR 457.2
Description: Mammalian Cell Extraction Kit for use in the research laboratory

Mammalian Cell Extraction Kit
K269-500	Biovision	each	EUR 352.8

Mammalian Cell Extraction Kit
K2114-500	ApexBio	500 assays	EUR 385.2

DNA Extraction Kit in Tubes
D100T	Cygnus Technologies	1 kit (50 tubes)	EUR 562.8
Description: DNA Extraction Kit in Tubes by Cygnus Technologies is available in Europe via Gentaur.

DNA Extraction Kit in Wells
D100W	Cygnus Technologies	1 kit (96 wells plate)	EUR 562.8
Description: DNA Extraction Kit in Wells by Cygnus Technologies is available in Europe via Gentaur.

Triton Extraction Buffer(TEB), for Histon Extraction
T8300-100	GenDepot	100ml	EUR 151.2

Triton Extraction Buffer(TEB), for Histon Extraction
T8300-500	GenDepot	500ml	EUR 170.4

Nuclear Protein Extraction Kit
BSP009	Bio Basic	50Preps	EUR 106.98

EpiQuik Nuclear Extraction Kit
OP-0002	EpiGentek	EUR 350.58 EUR 211.20	100 Assays 100 Extractions

EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit
P-2003	EpiGentek	EUR 1084.86 EUR 312.40 EUR 500.50 EUR 738.10	96 Reactions 24 Reactions 48 Reactions 96 Reactions

Membrane Protein Extraction Kit
55R-1357	Fitzgerald	50 assays	EUR 888
Description: Membrane Protein Extraction Kit for use in the research laboratory

Membrane Protein Extraction Kit
MPE01-12S	ProFoldin	0.5 ml	EUR 117.02
Description: This product includes 0.5 ml of 12 solutions for screening and micro-scale preparative extraction of membrane proteins (up to 1.5 mg) from the cell membranes.