ChromaFlash Chromatin Extraction Package

epigentek chromoplasts chromatin

Background Information
Chromatin immunoprecipitaton (ChIP) provides an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions could possibly be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could possibly be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Package deal is an entire set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle may be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly fast course of: your whole course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
  • Helpful and versatile: the bundle is suitable for getting ready every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.
epigentek chromoplasts chromatin
epigentek chromoplasts chromatin

 

Principle & Course of
The ChromaFlash Chromatin Extraction Package deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, modern and frozen tissues, and so forth. The amount of cells and tissues for each preparation could possibly be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 106  cells or per 50 mg tissues.

Background Information
Chromatin immunoprecipitation (ChIP) provides an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions could possibly be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could possibly be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.

Principle & Course of
The ChromaFlash Plant Chromatin Extraction Package deal accommodates the entire reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

miRNA Extraction Kit

K1456-50 50 Preps
EUR 661.2

ExoDNA? Extraction Kit

K1242-25 each
EUR 705.6

ExoDNA? Extraction Kit

K1242-50 each
EUR 1057.2

Up RNA Extraction Kit

20-abx098087
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit 

P-2002
  • EUR 1084.86
  • EUR 312.40
  • EUR 500.50
  • EUR 738.10
  • 96 Reactions
  • 24 Reactions
  • 48 Reactions
  • 96 Reactions

Quick Gel Extraction Kit

20-abx098085
  • EUR 343.20
  • EUR 243.60
  • 200 rxns
  • 50 rxns

Plant RNA extraction Kit

20-abx098088
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Chromatin protein Cren7 Antibody (HRP)

20-abx109204
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gel Extraction Kit (100 preps)

9K-006-0001 100preps
EUR 283.42

Gel Extraction Kit (200 preps)

9K-006-0002 200preps, 200prep
EUR 418.09

Chromatin protein Cren7 Antibody (FITC)

20-abx107787
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

FFPE Protein Extraction Kit

BSP058 50Preps
EUR 122.64

Exosomal RNA Extraction Kit

P200R,-part-of-P200 - Ask for price

Exosomal DNA Extraction Kit

P230-25 - Ask for price

Exosomal DNA Extraction Kit

P230-50 - Ask for price

Plant Tissue Extraction Kit

K296-50 each
EUR 548.4

Chromatin Modifying Protein 2A Protein

20-abx261989
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Chromatin Modifying Protein 2B Protein

20-abx262025
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Chromatin Modifying Protein 4A Protein

20-abx263203
  • EUR 276.00
  • EUR 1779.60
  • EUR 393.60
  • 1 µg
  • 50 ug
  • 5 ug

Chromatin Modifying Protein 1A Protein

20-abx263326
  • EUR 1930.80
  • EUR 393.60
  • EUR 276.00
  • 100 ug
  • 10 ug
  • 2 µg

Chromatin Protein Cren7 (creN7) Antibody

20-abx109437
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

ATRX, Chromatin Remodeler (ATRX) Antibody

20-abx325123
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

ATRX, Chromatin Remodeler (ATRX) Antibody

abx331300-100ul 100 ul
EUR 510

Total Protein Extraction Kit

BSP003 50Preps
EUR 130.99

Plant Protein Extraction Kit

BSP004 20Preps
EUR 133.08

Total Protein Extraction Kit

K3011010 1 kit
EUR 367

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

Up Plus RNA Extraction Kit

20-abx098093
  • EUR 444.00
  • EUR 126.00
  • 100 rxns
  • 2 rxns

Chromatin protein Cren7 Antibody (Biotin)

20-abx106375
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Mammalian Cell Extraction Kit

55R-1358 500 assays
EUR 457.2
Description: Mammalian Cell Extraction Kit for use in the research laboratory

Mammalian Cell Extraction Kit

K269-500 each
EUR 352.8

Mammalian Cell Extraction Kit

K2114-500 500 assays
EUR 385.2

DNA Extraction Kit in Tubes

D100T 1 kit (50 tubes)
EUR 562.8
Description: DNA Extraction Kit in Tubes by Cygnus Technologies is available in Europe via Gentaur.

DNA Extraction Kit in Wells

D100W 1 kit (96 wells plate)
EUR 562.8
Description: DNA Extraction Kit in Wells by Cygnus Technologies is available in Europe via Gentaur.

Triton Extraction Buffer(TEB), for Histon Extraction

T8300-100 100ml
EUR 151.2

Triton Extraction Buffer(TEB), for Histon Extraction

T8300-500 500ml
EUR 170.4

Nuclear Protein Extraction Kit

BSP009 50Preps
EUR 106.98

EpiQuik Nuclear Extraction Kit 

OP-0002
  • EUR 350.58
  • EUR 211.20
  • 100 Assays
  • 100 Extractions

EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit 

P-2003
  • EUR 1084.86
  • EUR 312.40
  • EUR 500.50
  • EUR 738.10
  • 96 Reactions
  • 24 Reactions
  • 48 Reactions
  • 96 Reactions

Membrane Protein Extraction Kit

55R-1357 50 assays
EUR 888
Description: Membrane Protein Extraction Kit for use in the research laboratory

Membrane Protein Extraction Kit

MPE01-12S 0.5 ml
EUR 117.02
Description: This product includes 0.5 ml of 12 solutions for screening and micro-scale preparative extraction of membrane proteins (up to 1.5 mg) from the cell membranes.