Crispr Nuclease Crispr Pam Sequence Crispr Plasmids Crispr Products Crispr Service Crispr Tutorial Custom Dna Synthesis Custom Gene Synthesis elisa Goat Hamster Insect Killifish Leech Listeria Orangutan Pig Pigeon Plant
Determining reliable parameter estimates for within-host and within-vector models of Zika virus
On this paper, we introduce three within-host and one within-vector fashions of Zika virus. The within-host fashions are the goal cell restricted mannequin, the goal cell restricted mannequin with pure killer (NK) cells class, and a within-host-within-fetus mannequin of a pregnant particular person. The within-vector mannequin consists of the Zika virus dynamics within the midgut and salivary glands.
The within-host fashions should not structurally identifiable with respect to knowledge on viral load and NK cell counts. After rescaling, the scaled within-host fashions are regionally structurally identifiable. The within-vector mannequin is structurally identifiable with respect to viremia knowledge within the midgut and salivary glands. Utilizing Monte Carlo Simulations, we discover that concentrate on cell restricted mannequin is virtually identifiable from knowledge on viremia; the goal cell restricted mannequin with NK cell class is virtually identifiable, apart from the rescaled half saturation fixed. The within-host-within-fetus mannequin has all fetus-related parameters not virtually identifiable with out knowledge on the fetus, in addition to the rescaled half saturation fixed can be not virtually identifiable. The remaining parameters are virtually identifiable. Lastly we discover that not one of the parameters of the within-vector mannequin is virtually identifiable.
Biodistribution of Adeno-Related Virus Serotype 5 Viral Vectors Following Intrathecal Injection
The pharmacokinetic profile of AAV particles following intrathecal supply has not but been clearly outlined. The current examine evaluated the distribution profile of adeno-associated virus serotype 5 (AAV5) viral vectors following lumbar intrathecal injection in mice. After a single bolus intrathecal injection, viral DNA concentrations in mouse entire blood, spinal twine, and peripheral tissues had been decided utilizing quantitative polymerase chain response (qPCR). The kinetics of AAV5 vector in entire blood and the focus over time in spinal and peripheral tissues had been analyzed. Distribution of the AAV5 vector to all ranges of the spinal twine, dorsal root ganglia, and into systemic circulation occurred quickly inside 30 min following injection. Vector focus in entire blood reached a most 6 h postinjection with a half-life of roughly 12 h. Space beneath the curve knowledge revealed the best focus of vector distributed to dorsal root ganglia tissue. Immunohistochemical evaluation revealed AAV5 particle colocalization with the pia mater on the spinal twine and macrophages within the dorsal root ganglia (DRG) 30 min after injection. These outcomes show the widespread distribution of AAV5 particles by means of cerebrospinal fluid and preferential focusing on of DRG tissue with potential clearance mechanisms by way of DRG macrophages.
Regulation of RNA Interference Pathways within the Insect Vector Laodelphax striatellus by Viral Proteins of Rice Stripe Virus
RNA interference (RNAi), particularly the small interfering RNA (siRNA) and microRNA (miRNA) pathways, performs an essential function in defending in opposition to viruses in vegetation and bugs. Nevertheless, how insect-transmitted phytoviruses regulate the RNAi-mediated antiviral response in vector bugs has barely been uncovered. On this examine, we explored the interplay between rice stripe virus (RSV) and the miRNA and siRNA pathways of the small brown planthopper, which is a vector insect.
The transcript and protein ranges of key genes within the two RNAi pathways didn’t change through the RSV an infection course of. When the expression of insect Ago1, Ago2, or Translin was silenced by the injection of double-stranded RNAs focusing on these genes, viral replication was promoted with Ago2 silencing however inhibited with Translin silencing. Protein-protein binding assays confirmed that viral NS2 and RNA-dependent RNA polymerase interacted with insect Ago2 and Translin, respectively.
When NS2 was knocked down, the transcript degree of Ago2 elevated and viral replication was inhibited. Subsequently, viral NS2 behaved like an siRNA suppressor in vector bugs. This protein-binding regulation of insect RNAi techniques displays a sophisticated and various coevolution of viruses with their vector bugs.
Adeno-Related Vector-Delivered CRISPR/ Sa Cas9 System Reduces Feline Leukemia Virus Manufacturing In Vitro
Feline leukemia virus (FeLV) is a retrovirus of cats worldwide. Excessive viral masses are related to progressive an infection and the demise of the host, resulting from FeLV-associated illness. In distinction, low viral masses, an efficient immune response, and a greater scientific consequence may be noticed in cats with regressive an infection. We hypothesize that by reducing viral masses in progressively contaminated cats, utilizing CRISPR/SaCas9-assisted gene remedy, the cat’s immune system could also be permitted to direct the an infection in direction of a regressive consequence.
In a step in direction of this aim, the current examine evaluates completely different adeno-associated vectors (AAVs) for his or her competence in delivering a gene enhancing system into feline cells, adopted by investigations of the CRISPR/SaCas9 focusing on effectivity for various websites throughout the FeLV provirus. 9 pure AAV serotypes, two AAV hybrid strains, and Anc80L65, an in silico predicted AAV ancestor, had been examined for his or her potential to contaminate completely different feline cell strains and feline major cells. AAV-DJ revealed superior an infection effectivity and was thus employed in subsequent transduction experiments.
The introduction of double-strand breaks, utilizing the CRISPR/SaCas9 system focusing on 12 chosen FeLV provirus websites, was confirmed by T7 endonuclease 1 (T7E1), in addition to Monitoring of Indels by Decomposition (TIDE) evaluation. The best proportion (as much as 80%) of nonhomologous end-joining (NHEJ) was discovered within the extremely conserved gag and pol areas. Subsequent transduction experiments, utilizing AAV-DJ, confirmed indel formation and confirmed a big discount in FeLV p27 antigen for some targets. The focusing on of the FeLV provirus was environment friendly when utilizing the CRISPR/SaCas9 strategy in vitro. Whether or not the noticed extent of provirus focusing on might be enough to supply progressively FeLV-infected cats with the means to beat the an infection must be additional investigated in vivo.
Nuances of Whitefly Vector-Crinivirus Interactions Revealed within the Foregut Retention and Transmission of Lettuce Chlorosis Virus by Two Bemisia tabaci Cryptic Species
Lettuce infectious yellows virus is the primary crinivirus for which the retention of purified virions ingested into the whitefly (Bemisia tabaci New World (NW)) vector’s foregut, has been demonstrated to be a requisite for profitable virus transmission. This key discovering helps the speculation that the determinant of foregut retention and transmission is current on the virion itself. Nevertheless, whether or not that is additionally true for different criniviruses has not been established. Right here, we offer proof that lettuce chlorosis virus (LCV) acquired from vegetation is retained within the foreguts of each the B. tabaci NW and Center East-Asia Minor 1 (MEAM1) vector species and transmitted upon inoculation feeding. An affiliation between foregut retention and transmission by NW vectors can be noticed following the acquisition and inoculation feeding of LCV virions purified utilizing a typical process involving 2% or 4% (v/v) Triton™ X-100 (TX-100). Nevertheless, whereas virions purified with 2% or 4% TX-100 are additionally retained within the foreguts of MEAM1 vectors, transmission is noticed with the 4% TX-100-purified virions or when extra vectors are used for acquisition and inoculation feeding. These outcomes recommend that an intrinsic distinction exists between NW and MEAM1 vectors of their interactions with, and transmission of, LCV virions.
 KIR2DL4 Antibody |
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| DF13591 | Affbiotech | 100ul | EUR 420 |
 Rabbit Polyclonal antibody Anti-CRBN |
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| Anti-CRBN | ImmunoStep | 50 µg | EUR 418.8 |
 Human KIR2DL4 shRNA Plasmid |
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| 20-abx952576 | Abbexa |
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 Human KIR2DL4 ELISA KIT |
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| ELI-21303h | Lifescience Market | 96 Tests | EUR 988.8 |
) KIR2DL4 Recombinant Protein (Human) |
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| RP017041 | ABM | 100 ug | Ask for price |
 KIR2DL4 Rabbit pAb |
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| A12836-100ul | Abclonal | 100 ul | EUR 369.6 |
KIR2DL4 Rabbit pAb |
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| A12836-200ul | Abclonal | 200 ul | EUR 550.8 |
KIR2DL4 Rabbit pAb |
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| A12836-20ul | Abclonal | 20 ul | EUR 219.6 |
KIR2DL4 Rabbit pAb |
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| A12836-50ul | Abclonal | 50 ul | EUR 267.6 |
 KIR2DL4 Blocking Peptide |
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| 33R-9831 | Fitzgerald | 100 ug | EUR 216 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of KIR2DL4 antibody, catalog no. 70R-2365 |
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 KIR2DL4 Polyclonal Antibody |
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| 27806-100ul | SAB | 100ul | EUR 302.4 |
KIR2DL4 Polyclonal Antibody |
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| 27806-50ul | SAB | 50ul | EUR 224.4 |
 KIR2DL4 Recombinant Protein |
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| 91-290 | ProSci | 0.05 mg | EUR 556.8 |
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Description: Killer cell immunoglobulin-like receptor 2DL4(KIR2DL4) is a Single-pass type I membrane protein and contains 2 Ig-like C2-type (immunoglobulin-like) domains.It belongs to the immunoglobulin superfamily. KIR2DL4 is expressed in all NK cells and some T cells. KIR2DL4 activates the cytotoxicity of NK cells, despite the presence of an immunoreceptor tyrosine-based inhibition motif (ITIM) in its cytoplasmic tail. The ITIM was not necessary for activation of lysis by KIR2DL4. The activation signal of KIR2DL4 was sensitive to inhibition by another ITIM-containing receptor. The activation-deficient mutant of KIR2DL4 inhibited the signal delivered by the activating receptor CD16. |
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 KIR2DL4 cloning plasmid |
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| CSB-CL857457HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the KIR2DL4 gene. |
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 pOTB7-KIR2DL4 Plasmid |
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| PVTB00348 | Lifescience Market | 2 ug | EUR 427.2 |
 (pORF)) KIR2DL4 ORF Vector (Human) (pORF) |
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| ORF005681 | ABM | 1.0 ug DNA | EUR 114 |
 KIR2DL4 Polyclonal Conjugated Antibody |
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| C27806 | SAB | 100ul | EUR 476.4 |
 pEGFP-flag-KIR2DL4 Plasmid |
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| PVTB00348-2a | Lifescience Market | 2 ug | EUR 427.2 |
) KIR2DL4 sgRNA CRISPR Lentivector set (Human) |
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| K1150301 | ABM | 3 x 1.0 ug | EUR 406.8 |
 Polyclonal Goat anti-GST α-form |
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| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 336 |
 Polyclonal Goat anti-GST μ-form |
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| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 336 |
 Polyclonal Goat anti-GST p-form |
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| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 336 |
 KIR2DL3 / KIR2DL1 / KIR2DL4 / KIR2DS4 Antibody |
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| 20-abx339417 | Abbexa |
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KIR2DL3 / KIR2DL1 / KIR2DL4 / KIR2DS4 Antibody |
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| 20-abx210854 | Abbexa |
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 KIR2DL3/KIR2DL1/KIR2DL4/KIR2DS4 Antibody |
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| 1-CSB-PA205578 | Cusabio |
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Description: A polyclonal antibody against KIR2DL3/KIR2DL1/KIR2DL4/KIR2DS4. Recognizes KIR2DL3/KIR2DL1/KIR2DL4/KIR2DS4 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200 |
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KIR2DL3/KIR2DL1/KIR2DL4/KIR2DS4 Antibody |
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| 1-CSB-PA246816 | Cusabio |
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Description: A polyclonal antibody against KIR2DL3/KIR2DL1/KIR2DL4/KIR2DS4. Recognizes KIR2DL3/KIR2DL1/KIR2DL4/KIR2DS4 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:50-1:100 |
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) Recombinant Human KIR2DL4/CD158d/KIR103 (C-6His) |
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| C310-10ug | Novoprotein | 10ug | EUR 157.2 |
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Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
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Recombinant Human KIR2DL4/CD158d/KIR103 (C-6His) |
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| C310-1mg | Novoprotein | 1mg | EUR 2739.6 |
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Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
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Recombinant Human KIR2DL4/CD158d/KIR103 (C-6His) |
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| C310-500ug | Novoprotein | 500ug | EUR 1935.6 |
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Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
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Recombinant Human KIR2DL4/CD158d/KIR103 (C-6His) |
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| C310-50ug | Novoprotein | 50ug | EUR 327.6 |
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Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
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 (Target 1)) KIR2DL4 sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K1150302 | ABM | 1.0 ug DNA | EUR 184.8 |
 (Target 2)) KIR2DL4 sgRNA CRISPR Lentivector (Human) (Target 2) |
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| K1150303 | ABM | 1.0 ug DNA | EUR 184.8 |
 (Target 3)) KIR2DL4 sgRNA CRISPR Lentivector (Human) (Target 3) |
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| K1150304 | ABM | 1.0 ug DNA | EUR 184.8 |
 (pPB-C-His)) KIR2DL4 Protein Vector (Human) (pPB-C-His) |
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| PV022721 | ABM | 500 ng | EUR 394.8 |
 (pPB-N-His)) KIR2DL4 Protein Vector (Human) (pPB-N-His) |
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| PV022722 | ABM | 500 ng | EUR 394.8 |
 (pPM-C-HA)) KIR2DL4 Protein Vector (Human) (pPM-C-HA) |
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| PV022723 | ABM | 500 ng | EUR 394.8 |
 (pPM-C-His)) KIR2DL4 Protein Vector (Human) (pPM-C-His) |
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| PV022724 | ABM | 500 ng | EUR 394.8 |
 Recombinant Human KIR2DL4 Protein, His, Insect-1ug |
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| QP12489-1ug | EnQuireBio | 1ug | EUR 186 |
 Recombinant Human KIR2DL4 Protein, His, Insect-50ug |
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| QP12489-50ug | EnQuireBio | 50ug | EUR 1513.2 |
 Recombinant Human KIR2DL4 Protein, His, Insect-5ug |
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| QP12489-5ug | EnQuireBio | 5ug | EUR 241.2 |
 KIR2DL4 3'UTR GFP Stable Cell Line |
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| TU061796 | ABM | 1.0 ml | EUR 1825.2 |
 KIR2DL4 3'UTR Luciferase Stable Cell Line |
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| TU011796 | ABM | 1.0 ml | EUR 1825.2 |
) KIR2DL4 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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| K1150305 | ABM | 3 x 1.0 ug | EUR 451.2 |
 Antibody) Killer Cell Immunoglobulin-Like Receptor 2DL4 (KIR2DL4) Antibody |
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| abx145740-100ug | Abbexa | 100 ug | EUR 469.2 |
 (Target 1)) KIR2DL4 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1) |
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| K1150306 | ABM | 1.0 ug DNA | EUR 200.4 |
 (Target 2)) KIR2DL4 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2) |
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| K1150307 | ABM | 1.0 ug DNA | EUR 200.4 |
 (Target 3)) KIR2DL4 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) |
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| K1150308 | ABM | 1.0 ug DNA | EUR 200.4 |
 KIR2DL4 Killer Cell Immunoglobulin-Like Receptor, 2 Domains Long Cytoplasmic Tail 4 Human Recombinant Protein |
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| PROTQ99706 | BosterBio | Regular: 5ug | EUR 380.4 |
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Description: KIR2DL4 Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 458 amino acids (24-242 a.a.) and having a molecular mass of 51kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). KIR2DL4 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques. |
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 anti-human CCR1 |
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| 20R-3028 | Fitzgerald | 200 ug | EUR 704.4 |
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Description: Goat anti-human CCR1 antibody |
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 anti-human RecQL4 |
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| AR05-PA0007 | Abfrontier | 100 ul | EUR 400.8 |
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Description: Rabbit polyclonal to human RecQL4 |
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 Anti-Human IgG |
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| DB-173-0.1 | DB Biotech | 100 μl | EUR 254.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-173-0.2 | DB Biotech | 200 μl | EUR 357.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-173-0.5 | DB Biotech | 500 μl | EUR 460.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-173-1 | DB Biotech | 1 ml | EUR 735.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-173-RTU-15 | DB Biotech | 15 ml | EUR 426 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Human IgG |
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| DB-173-RTU-7 | DB Biotech | 7 ml | EUR 277.2 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Human IgG |
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| DB-174-0.1 | DB Biotech | 100 μl | EUR 254.4 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-174-0.2 | DB Biotech | 200 μl | EUR 357.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-174-0.5 | DB Biotech | 500 μl | EUR 460.8 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-174-1 | DB Biotech | 1 ml | EUR 735.6 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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