IPREM/LCABIE Laboratoire de Chimie Analytique Bio-inorganique et Environnement

PRINCIPLE & PROCEDURE

The EpiQuik  Circulating Full Histone H3 Quantification Package deal (Colorimetric) is designed for measuring entire histone H3 in plasma or serum. In an assay with this gear, the histone H3 proteins inside the plasma/serum sample are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade enchancment reagent. The ratio of histone H3 is proportional to the depth of absorbance. Completely the amount of H3 may be quantitated by evaluating to the standard administration.

What’s DNA Methylation?

DNA methylation occurs by the covalent addition of a methyl group (CH³) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5-mC). When a CpG island inside the promoter space of a gene is methylated, expression of the gene is repressed. Quite a lot of human sicknesses akin to most cancers occur when DNA methylation is not going to be appropriately established and/or maintained.

DNA methylation is a dynamic and reversible course of that may result in quite a few intermediate modification varieties. 5-mC remodeled from cytosine by DNMTs may be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is extra modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the equivalent TET enzymes. Analysis of DNA methylation is essential to understanding and concentrating on DNA methylation–associated changes, which could help decide DNA methylation markers and their natural regulation.

Which DNA Methylation Machine is Correct for Your Experiment?

There are a variety of methods accessible for profiling genome-wide and region-specific DNA methylation. Although worth, minimal sample enter requirements, accuracy, rapidity, and throughput are important considerations, choosing the right method is most important for the success of the analysis.

Sooner than you begin, we advise reviewing any literature that exists related to your proposed experiment. If totally different researchers have already achieved the legwork, you might as properly reap the advantages of this accessible data as a base to assemble your analysis spherical. You and your crew can then make use of variations and distinctive angles to make the analysis completely totally different and attention-grabbing, reviewing the beforehand achieved work fastidiously to find out potential obstacles or points that the sooner researchers encountered.

For those who’re unsure which method will work in your analysis, we advise starting with a simple pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the utilization of a fast world DNA methylation or hydroxymethylation assay, you’ll assess whether or not or not your sample accommodates 5-mC or 5-hmC and the best way lots. Then you’ll determine the next step and which method is appropriate to utilize. 2) For individuals who uncover your sample accommodates every 5-mC and 5-hmC, oxidative bisulfite-sequencing might very nicely be used.

 

This system can decide and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base resolution. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS may be carried out. (3) If the sample amount is restricted or single-cell diploma analysis is desired, post-bisulfite sequencing might meet the requirement. (4) For analyzing methylation/hydroxymethylation of specific gene panels akin to a most cancers gene panel or quite a few genomic areas, centered bisulfite-sequencing or centered oxidative bisulfite-sequencing will work.

Methylated DNA Quantification

DNA methylation performs a vital operate in common organismal enchancment and cellular differentiation in bigger organisms. Gene expression and the occasion of just about all sorts of most cancers are moreover tied to DNA methylation. For example, world decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going introduced on by methyl-deficiency because of a variety of environmental influences and has been proposed as a molecular marker in quite a few natural processes akin to most cancers. World quantification of DNA methylation is important for understanding the roles that gene expression and silencing play in most cancers enchancment and totally different sicknesses.

 

BOK Peptide
3357P	ProSci	0.05 mg	EUR 197.7
Description: (NT) BOK peptide

Bok antibody
20R-1439	Fitzgerald	100 ug	EUR 781.2
Description: Rabbit polyclonal Bok antibody

BOK Antibody
24253-100ul	SAB	100ul	EUR 468

BOK Antibody
ABD3829	Lifescience Market	100 ug	EUR 525.6

BOK siRNA
20-abx900655	Abbexa	EUR 661.20 EUR 878.40	15 nmol 30 nmol

BOK siRNA
20-abx909215	Abbexa	EUR 661.20 EUR 878.40	15 nmol 30 nmol

BOK siRNA
20-abx909216	Abbexa	EUR 661.20 EUR 878.40	15 nmol 30 nmol

BOK Antibody
1-CSB-PA892353LA01HU	Cusabio	EUR 380.40 EUR 402.00	100ug 50ug
Description: A polyclonal antibody against BOK. Recognizes BOK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

BOK Antibody
1-CSB-PA904266	Cusabio	EUR 380.40 EUR 292.80	100ul 50ul
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

BOK Antibody
DF3829	Affbiotech	200ul	EUR 420

BOK Antibody
1-CSB-PA437965	Cusabio	EUR 380.40 EUR 292.80	100ul 50ul
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:20-1:100

Bok Antibody
3043-100	Biovision	each	EUR 430.8

Automatic CO2 Change Over Unit
INC6176	Scientific Laboratory Supplies	EACH	EUR 1288.2

Forceps Dissecting Turn Over End
D02129	Scientific Laboratory Supplies	EACH	EUR 4.67

Bolle TG10 Safety Over Glasses
SAF1106	Scientific Laboratory Supplies	EACH	EUR 8.39

Gas Change Over Unit 30Psi
GAS1000	Scientific Laboratory Supplies	EACH	EUR 904.02

Gas Change Over Unit 60Psi
GAS1002	Scientific Laboratory Supplies	EACH	EUR 905.16

Gas Change Over Unit 100Psi
GAS1004	Scientific Laboratory Supplies	EACH	EUR 922.26

BOK Polyclonal Antibody
29816-100ul	SAB	100ul	EUR 302.4

BOK Polyclonal Antibody
29816-50ul	SAB	50ul	EUR 224.4

BOK Polyclonal Antibody
ABP57917-003ml	Abbkine	0.03ml	EUR 189.6
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90

BOK Polyclonal Antibody
ABP57917-01ml	Abbkine	0.1ml	EUR 346.8
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90

BOK Polyclonal Antibody
ABP57917-02ml	Abbkine	0.2ml	EUR 496.8
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90

BOK Rabbit pAb
A16354-100ul	Abclonal	100 ul	EUR 369.6

BOK Rabbit pAb
A16354-200ul	Abclonal	200 ul	EUR 550.8

BOK Rabbit pAb
A16354-20ul	Abclonal	20 ul	EUR 219.6

BOK Rabbit pAb
A16354-50ul	Abclonal	50 ul	EUR 267.6

Polyclonal Bok Antibody
APR00010G	Leading Biology	0.1mg	EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Bok . This antibody is tested and proven to work in the following applications:

Polyclonal BOK Antibody
APR06350G	Leading Biology	0.1 mg	EUR 790.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BOK . This antibody is tested and proven to work in the following applications: