
EpiGentek | Assay Kits, Antibodies
PRINCIPLE & PROCEDURE
The EpiQuik Circulating Full Histone H3 Quantification Package deal (Colorimetric) is designed for measuring entire histone H3 in plasma or serum. In an assay with this gear, the histone H3 proteins inside the plasma/serum sample are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade enchancment reagent. The ratio of histone H3 is proportional to the depth of absorbance. Completely the amount of H3 may be quantitated by evaluating to the standard administration.
What’s DNA Methylation?
DNA methylation occurs by the covalent addition of a methyl group (CH3) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5-mC). When a CpG island inside the promoter space of a gene is methylated, expression of the gene is repressed. Quite a lot of human sicknesses akin to most cancers occur when DNA methylation is not going to be appropriately established and/or maintained.
DNA methylation is a dynamic and reversible course of that may result in quite a few intermediate modification varieties. 5-mC remodeled from cytosine by DNMTs may be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is extra modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the equivalent TET enzymes. Analysis of DNA methylation is essential to understanding and concentrating on DNA methylation–associated changes, which could help decide DNA methylation markers and their natural regulation.
Which DNA Methylation Machine is Correct for Your Experiment?
There are a variety of methods accessible for profiling genome-wide and region-specific DNA methylation. Although worth, minimal sample enter requirements, accuracy, rapidity, and throughput are important considerations, choosing the right method is most important for the success of the analysis.
Sooner than you begin, we advise reviewing any literature that exists related to your proposed experiment. If totally different researchers have already achieved the legwork, you might as properly reap the advantages of this accessible data as a base to assemble your analysis spherical. You and your crew can then make use of variations and distinctive angles to make the analysis completely totally different and attention-grabbing, reviewing the beforehand achieved work fastidiously to find out potential obstacles or points that the sooner researchers encountered.
For those who’re unsure which method will work in your analysis, we advise starting with a simple pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the utilization of a fast world DNA methylation or hydroxymethylation assay, you’ll assess whether or not or not your sample accommodates 5-mC or 5-hmC and the best way lots. Then you’ll determine the next step and which method is appropriate to utilize. 2) For individuals who uncover your sample accommodates every 5-mC and 5-hmC, oxidative bisulfite-sequencing might very nicely be used.
This system can decide and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base resolution. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS may be carried out. (3) If the sample amount is restricted or single-cell diploma analysis is desired, post-bisulfite sequencing might meet the requirement. (4) For analyzing methylation/hydroxymethylation of specific gene panels akin to a most cancers gene panel or quite a few genomic areas, centered bisulfite-sequencing or centered oxidative bisulfite-sequencing will work.

Methylated DNA Quantification
DNA methylation performs a vital operate in common organismal enchancment and cellular differentiation in bigger organisms. Gene expression and the occasion of just about all sorts of most cancers are moreover tied to DNA methylation. For example, world decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going introduced on by methyl-deficiency because of a variety of environmental influences and has been proposed as a molecular marker in quite a few natural processes akin to most cancers. World quantification of DNA methylation is important for understanding the roles that gene expression and silencing play in most cancers enchancment and totally different sicknesses.
![]() BOK Antibody |
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1-CSB-PA904266 | Cusabio |
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Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100 |
![]() BOK Antibody |
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1-CSB-PA892353LA01HU | Cusabio |
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Description: A polyclonal antibody against BOK. Recognizes BOK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
![]() BOK Antibody |
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DF3829 | Affbiotech | 200ul | EUR 304 |
Description: BOK Antibody detects endogenous levels of total BOK. |
![]() BOK Antibody |
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ABD3829 | Lifescience Market | 100 ug | EUR 438 |
![]() Bok Antibody |
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3043-100 | Biovision | EUR 359 |
![]() Bok antibody |
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20R-1439 | Fitzgerald | 100 ug | EUR 651 |
Description: Rabbit polyclonal Bok antibody |
![]() BOK Antibody |
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3357-002mg | ProSci | 0.02 mg | EUR 171.82 |
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation. |
![]() BOK Antibody |
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3357-01mg | ProSci | 0.1 mg | EUR 436.42 |
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation. |
![]() Bok antibody |
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70R-11577 | Fitzgerald | 100 ug | EUR 447 |
Description: Rabbit polyclonal Bok antibody |
![]() BOK Peptide |
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3357P | ProSci | 0.05 mg | EUR 164.75 |
Description: (NT) BOK peptide |
![]() BOK Peptide |
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42-325P | ProSci | 0.1 mg | EUR 338 |
Description: (IN) BOK Peptide |
![]() HEK-293T Telomerase Over-Expressing Cell Pellet |
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abx069991-1Pellet | Abbexa | 1 Pellet | EUR 398 |
![]() Bradford reagent |
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BDE641 | Bio Basic | 100ml | EUR 61.01 |
![]() Beaucage reagent |
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HY-100951 | MedChemExpress | 10mM/1mL | EUR 126 |
![]() BOP reagent |
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5-02141 | CHI Scientific | 25g | Ask for price |
![]() BOP reagent |
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5-02142 | CHI Scientific | 100g | Ask for price |
![]() Bluing Reagent |
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BRT030 | ScyTek Laboratories | 30 ml | EUR 60 |
![]() Bluing Reagent |
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BRT125 | ScyTek Laboratories | 125 ml | EUR 63 |
![]() Bluing Reagent |
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BRT3800 | ScyTek Laboratories | 1 Gal. | EUR 184 |
![]() Bluing Reagent |
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BRT500 | ScyTek Laboratories | 500 ml | EUR 76 |
![]() Bluing Reagent |
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BRT999 | ScyTek Laboratories | 1000 ml | EUR 88 |
![]() BOP reagent |
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A7015-100000 | ApexBio | 100 g | EUR 200 |
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
![]() BOP reagent |
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A7015-25000 | ApexBio | 25 g | EUR 113 |
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
![]() Traut's Reagent |
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2330-1000 | Biovision | EUR 349 |
![]() Traut's Reagent |
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2330-500 | Biovision | EUR 207 |
![]() MTS Reagent |
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2808-1000 | Biovision | EUR 990 |
![]() MTS Reagent |
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2808-250 | Biovision | EUR 365 |
![]() MTT Reagent |
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2809-1G | Biovision | EUR 180 |
![]() MTT Reagent |
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2809-5G | Biovision | EUR 544 |