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EpiGentek | Assay Kits, Antibodies

PRINCIPLE & PROCEDURE

The EpiQuik  Circulating Full Histone H3 Quantification Package deal (Colorimetric) is designed for measuring entire histone H3 in plasma or serum. In an assay with this gear, the histone H3 proteins inside the plasma/serum sample are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade enchancment reagent. The ratio of histone H3 is proportional to the depth of absorbance. Completely the amount of H3 may be quantitated by evaluating to the standard administration.

What’s DNA Methylation?

DNA methylation occurs by the covalent addition of a methyl group (CH3) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5-mC). When a CpG island inside the promoter space of a gene is methylated, expression of the gene is repressed. Quite a lot of human sicknesses akin to most cancers occur when DNA methylation is not going to be appropriately established and/or maintained.

DNA methylation is a dynamic and reversible course of that may result in quite a few intermediate modification varieties. 5-mC remodeled from cytosine by DNMTs may be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is extra modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the equivalent TET enzymes. Analysis of DNA methylation is essential to understanding and concentrating on DNA methylation–associated changes, which could help decide DNA methylation markers and their natural regulation.

Which DNA Methylation Machine is Correct for Your Experiment?

There are a variety of methods accessible for profiling genome-wide and region-specific DNA methylation. Although worth, minimal sample enter requirements, accuracy, rapidity, and throughput are important considerations, choosing the right method is most important for the success of the analysis.

Sooner than you begin, we advise reviewing any literature that exists related to your proposed experiment. If totally different researchers have already achieved the legwork, you might as properly reap the advantages of this accessible data as a base to assemble your analysis spherical. You and your crew can then make use of variations and distinctive angles to make the analysis completely totally different and attention-grabbing, reviewing the beforehand achieved work fastidiously to find out potential obstacles or points that the sooner researchers encountered.

For those who’re unsure which method will work in your analysis, we advise starting with a simple pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the utilization of a fast world DNA methylation or hydroxymethylation assay, you’ll assess whether or not or not your sample accommodates 5-mC or 5-hmC and the best way lots. Then you’ll determine the next step and which method is appropriate to utilize. 2) For individuals who uncover your sample accommodates every 5-mC and 5-hmC, oxidative bisulfite-sequencing might very nicely be used.

 

This system can decide and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base resolution. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS may be carried out. (3) If the sample amount is restricted or single-cell diploma analysis is desired, post-bisulfite sequencing might meet the requirement. (4) For analyzing methylation/hydroxymethylation of specific gene panels akin to a most cancers gene panel or quite a few genomic areas, centered bisulfite-sequencing or centered oxidative bisulfite-sequencing will work.

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Methylated DNA Quantification

DNA methylation performs a vital operate in common organismal enchancment and cellular differentiation in bigger organisms. Gene expression and the occasion of just about all sorts of most cancers are moreover tied to DNA methylation. For example, world decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going introduced on by methyl-deficiency because of a variety of environmental influences and has been proposed as a molecular marker in quite a few natural processes akin to most cancers. World quantification of DNA methylation is important for understanding the roles that gene expression and silencing play in most cancers enchancment and totally different sicknesses.

 

BOK Antibody
1-CSB-PA904266
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

BOK Antibody
1-CSB-PA892353LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against BOK. Recognizes BOK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

BOK Antibody
DF3829 200ul
EUR 304
Description: BOK Antibody detects endogenous levels of total BOK.

BOK Antibody
ABD3829 100 ug
EUR 438

Bok Antibody
3043-100
EUR 359

Bok antibody
20R-1439 100 ug
EUR 651
Description: Rabbit polyclonal Bok antibody

BOK Antibody
3357-002mg 0.02 mg
EUR 171.82
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation.

BOK Antibody
3357-01mg 0.1 mg
EUR 436.42
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation.

Bok antibody
70R-11577 100 ug
EUR 447
Description: Rabbit polyclonal Bok antibody

BOK Peptide
3357P 0.05 mg
EUR 164.75
Description: (NT) BOK peptide

BOK Peptide
42-325P 0.1 mg
EUR 338
Description: (IN) BOK Peptide

HEK-293T Telomerase Over-Expressing Cell Pellet
abx069991-1Pellet 1 Pellet
EUR 398

Bradford reagent
BDE641 100ml
EUR 61.01

Beaucage reagent
HY-100951 10mM/1mL
EUR 126

BOP reagent
5-02141 25g Ask for price

BOP reagent
5-02142 100g Ask for price

Bluing Reagent
BRT030 30 ml
EUR 60

Bluing Reagent
BRT125 125 ml
EUR 63

Bluing Reagent
BRT3800 1 Gal.
EUR 184

Bluing Reagent
BRT500 500 ml
EUR 76

Bluing Reagent
BRT999 1000 ml
EUR 88

BOP reagent
A7015-100000 100 g
EUR 200
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.

BOP reagent
A7015-25000 25 g
EUR 113
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.

Traut's Reagent
2330-1000
EUR 349

Traut's Reagent
2330-500
EUR 207

MTS Reagent
2808-1000
EUR 990

MTS Reagent
2808-250
EUR 365

MTT Reagent
2809-1G
EUR 180

MTT Reagent
2809-5G
EUR 544
Tags:
bioassay