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EpiGentek | Assay Kits, Antibodies

PRINCIPLE & PROCEDURE

The EpiQuik  Circulating Full Histone H3 Quantification Package deal (Colorimetric) is designed for measuring entire histone H3 in plasma or serum. In an assay with this gear, the histone H3 proteins inside the plasma/serum sample are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade enchancment reagent. The ratio of histone H3 is proportional to the depth of absorbance. Completely the amount of H3 may be quantitated by evaluating to the standard administration.

What’s DNA Methylation?

DNA methylation occurs by the covalent addition of a methyl group (CH3) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5-mC). When a CpG island inside the promoter space of a gene is methylated, expression of the gene is repressed. Quite a lot of human sicknesses akin to most cancers occur when DNA methylation is not going to be appropriately established and/or maintained.

DNA methylation is a dynamic and reversible course of that may result in quite a few intermediate modification varieties. 5-mC remodeled from cytosine by DNMTs may be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is extra modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the equivalent TET enzymes. Analysis of DNA methylation is essential to understanding and concentrating on DNA methylation–associated changes, which could help decide DNA methylation markers and their natural regulation.

Which DNA Methylation Machine is Correct for Your Experiment?

There are a variety of methods accessible for profiling genome-wide and region-specific DNA methylation. Although worth, minimal sample enter requirements, accuracy, rapidity, and throughput are important considerations, choosing the right method is most important for the success of the analysis.

Sooner than you begin, we advise reviewing any literature that exists related to your proposed experiment. If totally different researchers have already achieved the legwork, you might as properly reap the advantages of this accessible data as a base to assemble your analysis spherical. You and your crew can then make use of variations and distinctive angles to make the analysis completely totally different and attention-grabbing, reviewing the beforehand achieved work fastidiously to find out potential obstacles or points that the sooner researchers encountered.

For those who’re unsure which method will work in your analysis, we advise starting with a simple pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the utilization of a fast world DNA methylation or hydroxymethylation assay, you’ll assess whether or not or not your sample accommodates 5-mC or 5-hmC and the best way lots. Then you’ll determine the next step and which method is appropriate to utilize. 2) For individuals who uncover your sample accommodates every 5-mC and 5-hmC, oxidative bisulfite-sequencing might very nicely be used.

 

This system can decide and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base resolution. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS may be carried out. (3) If the sample amount is restricted or single-cell diploma analysis is desired, post-bisulfite sequencing might meet the requirement. (4) For analyzing methylation/hydroxymethylation of specific gene panels akin to a most cancers gene panel or quite a few genomic areas, centered bisulfite-sequencing or centered oxidative bisulfite-sequencing will work.

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Methylated DNA Quantification

DNA methylation performs a vital operate in common organismal enchancment and cellular differentiation in bigger organisms. Gene expression and the occasion of just about all sorts of most cancers are moreover tied to DNA methylation. For example, world decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going introduced on by methyl-deficiency because of a variety of environmental influences and has been proposed as a molecular marker in quite a few natural processes akin to most cancers. World quantification of DNA methylation is important for understanding the roles that gene expression and silencing play in most cancers enchancment and totally different sicknesses.

 

BOK Antibody
1-CSB-PA892353LA01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against BOK. Recognizes BOK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

BOK Antibody
1-CSB-PA904266
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

Bok antibody
70R-11577 100 ug
EUR 536.4
Description: Rabbit polyclonal Bok antibody

BOK Peptide
42-325P 0.1 mg
EUR 405.6
Description: (IN) BOK Peptide

BOK Antibody
24253-100ul 100ul
EUR 468

BOK Antibody
3357-002mg 0.02 mg
EUR 206.18
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation.

BOK Antibody
3357-01mg 0.1 mg
EUR 523.7
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation.

BOK Peptide
3357P 0.05 mg
EUR 197.7
Description: (NT) BOK peptide

Bok antibody
20R-1439 100 ug
EUR 781.2
Description: Rabbit polyclonal Bok antibody

Bok Antibody
3043-100
EUR 430.8

BOK Antibody
ABD3829 100 ug
EUR 525.6

BOK siRNA
20-abx900655
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

EP Reagent Biuret Reagent
1011601 1L
EUR 42.18

EP Reagent Iodoplatinate Reagent
1046300 200ML
EUR 360.24

EP Reagent Methoxyphenylacetic Reagent
1053601 100ML
EUR 354.54

EP Reagent Molybdovanadic Reagent
1056700 100ML
EUR 42.18

EP Reagent Phosphomolybdotungstic Reagent
1065000 100ML
EUR 161.88

Gas Change Over Unit 30Psi
GAS1000 EACH
EUR 904.02

Gas Change Over Unit 60Psi
GAS1002 EACH
EUR 905.16

Gas Change Over Unit 100Psi
GAS1004 EACH
EUR 922.26

Automatic CO2 Change Over Unit
INC6176 EACH
EUR 1288.2

Bolle TG10 Safety Over Glasses
SAF1106 EACH
EUR 8.39

Forceps Dissecting Turn Over End
D02129 EACH
EUR 4.67

BOK Polyclonal Antibody
ABP57917-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90

BOK Polyclonal Antibody
ABP57917-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90

BOK Polyclonal Antibody
ABP57917-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90

BOK Blocking Peptide
DF3829-BP 1mg
EUR 234

BOK Polyclonal Antibody
ES9443-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against BOK from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

BOK Polyclonal Antibody
ES9443-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against BOK from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA
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bioassay