IPREM/LCABIE Laboratoire de Chimie Analytique Bio-inorganique et Environnement

Pathogenic bacteria and virusses

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Factors Determining Transmission of Persistent Viruses by Bemisia tabaci and Emergence of New Virus-Vector Relationships

Many plant viruses depend upon insect vectors for his or her transmission and dissemination. The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is without doubt one of the most essential virus vectors, transmitting greater than 4 hundred virus species, the bulk belonging to begomoviruses (Geminiviridae), with their ssDNA genomes. Begomoviruses are transmitted by B. tabaci in a persistent, circulative method, throughout which the virus breaches limitations within the digestive, hemolymph, and salivary techniques, and interacts with insect proteins alongside the transmission pathway.
These interactions and the tissue tropism within the vector physique decide the effectivity and specificity of the transmission. This assessment describes the mechanisms concerned in circulative begomovirus transmission by B. tabaci, specializing in essentially the most studied virus on this regard, particularly the tomato yellow leaf curl virus (TYLCV) and its carefully associated isolates.
Moreover, the assessment goals at drawing consideration to the current knowhow of unorthodox virus-B. tabaci interactions. The current data of whitefly-mediated transmission of two recombinant poleroviruses (Luteoviridae), a virus group with an ssRNA genome and identified to be strictly transmitted with aphids, is mentioned with its broader context within the emergence of latest whitefly-driven virus illnesses.

Security and Immunogenicity of a Newcastle Illness Virus Vector-Based mostly SARS-CoV-2 Vaccine Candidate, AVX/COVID-12-HEXAPRO (Patria), in Pigs

Vaccines in opposition to extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) had been developed in report time and present wonderful efficacy and effectiveness in opposition to coronavirus illness 2019 (COVID-19). Nevertheless, at present authorised vaccines can not meet the worldwide demand. As well as, not one of the at present used vaccines is run intranasally to doubtlessly induce mucosal immunity.
Right here, we examined the security and immunogenicity of a second-generation SARS-CoV-2 vaccine that features a stabilized spike antigen and may be administered intranasally. The vaccine is predicated on a dwell Newcastle illness virus vector expressing a SARS-CoV-2 spike protein stabilized in a prefusion conformation with six helpful proline substitutions (AVX/COVID-12-HEXAPRO; Patria).
Immunogenicity testing within the pig mannequin confirmed that each intranasal and intramuscular software of the vaccine in addition to a mix of the 2 induced robust serum neutralizing antibody responses. Moreover, substantial reactivity to B.1.1.7, B.1.351, and P.1 spike variants was detected. Lastly, no opposed reactions had been discovered within the experimental animals at any dose degree or supply route. These outcomes point out that the experimental vaccine AVX/COVID-12-HEXAPRO (Patria) is secure and extremely immunogenic within the pig mannequin.
 IMPORTANCE A number of extremely efficacious vaccines for SARS-CoV-2 have been developed and are used within the inhabitants. Nevertheless, the present manufacturing capability can not meet the worldwide demand. Subsequently, extra vaccines-especially ones that may be produced domestically and at low cost-are urgently wanted. This work describes preclinical testing of a SARS-CoV-2 vaccine candidate which meets these standards.

 

Manufacturing of Modified Vaccinia Ankara Virus by Intensified Cell Cultures: A Comparability of Platform Applied sciences for Viral Vector Manufacturing

 

Modified Vaccinia Ankara (MVA) virus is a promising vector for vaccination in opposition to numerous difficult pathogens or the therapy of some sorts of cancers. As a result of this vector is unable to copy in human recipients, a excessive quantity of virions per dose is required for vaccination and gene remedy. Upstream course of intensification combining perfusion applied sciences, the avian suspension cell line AGE1.CR.pIX and the virus pressure MVA-CR19 is an choice to acquire very excessive MVA yields.

Right here we in contrast totally different choices for cell retention in perfusion mode utilizing typical stirred-tank bioreactors together with an alternating tangential circulation filtration system, an acoustic settler and an inclined settler. The final two choices allowed steady MVA virus harvesting. Moreover, we studied hollow-fiber primarily based bioreactors and an orbital-shaken bioreactor in perfusion mode, each out there for single-use. Productiveness for the virus pressure MVA-CR19 was in comparison with outcomes from batch and steady manufacturing reported in literature.

Our outcomes reveal that MVA virus is extremely secure at 37°C in cell tradition in order that cell retention units are solely required to maximise cell focus however not for steady harvesting. Utilizing a stirred-tank bioreactor, a perfusion technique throughout the entire run with working quantity growth after virus an infection resulted within the highest yields.

General, infectious MVA virus titers of two.1-16.5 × 109 virions/mL had been achieved in these intensified processes. Taken collectively, the research reveals a novel perspective on high-yield MVA virus manufacturing in typical bioreactor techniques linked to varied cell retention units and addresses choices for course of intensification together with totally single-use perfusion platforms. This text is protected by copyright. All rights reserved.

Mosaic Recombinant Adeno-associated Virus Vector rAAV/DJ/CAG for Focused Gene Supply to Melanoma Cells Metastasized to the Lung

 

Background/intention: Sufferers with metastasized melanoma have restricted therapy choices and poor prognosis. Subsequently, the event of remedies requires a brand new therapeutic strategy, of which gene remedy utilizing rAAV vectors may be proposed. The intention of the research was to look at the effectivity of the rAAV vector to transduce mouse melanoma cells each in vitro and in vivo.

Supplies and strategies: Completely different rAAV serotypes encoding GFP below the management of each rooster beta-actin and cytomegalovirus promoters had been used within the experiments. Intranasal, intraperitoneal, intravenous and intratumoral pathways of administration of rAAV vectors had been examined utilizing quantitative-PCR and immunohistochemical staining.

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Outcomes: The very best transduction effectivity in metastatic cells in vivo was noticed 7 days after intranasal administration of a 1010 gc/0.03 ml dose of rAAV/DJ-CAG.

 

Conclusion: Melanoma gene remedy primarily based on rAAV vectors is a potential therapy possibility.

Vector Competence of Aedes aegypti, Aedes albopictus and Culex quinquefasciatus from Brazil and New Caledonia for Three Zika Virus Lineages

 

Zika virus (ZIKV) has brought about extreme epidemics in South America starting in 2015, following its unfold by way of the Pacific. We comparatively assessed the vector competence of ten populations of Aedes aegypti and Ae. albopictus from Brazil and two of Ae. aegypti and considered one of Culex quinquefasciatus from New Caledonia to transmit three ZIKV isolates belonging to African, Asian and American lineages.

Not too long ago colonized mosquitoes from eight distinct websites from each nations had been orally challenged with the identical viral load (107 TCID50/mL) and examined after 7, 14 and 21 days. Cx. quinquefasciatus was refractory to an infection with all virus strains. In distinction, though competence diverse with geographical origin, Brazilian and New Caledonian Ae. aegypti may transmit the three ZIKV lineages, with a robust benefit for the African lineage (the one one reaching saliva one-week after problem).

Brazilian Ae. albopictus populations had been much less competent than Ae. aegypti populations. Ae. albopictus usually exhibited virtually no transmission for Asian and American lineages, however was environment friendly in transmitting the African ZIKV. Viral surveillance and mosquito management measures have to be strengthened to keep away from the unfold of latest ZIKV lineages and reduce the transmission of viruses at present circulating.

Anti-IL-8 antibody
STJ98175 100 µl
EUR 280.8
Description: Mouse monoclonal to IL-8.
Anti-IL-8 antibody
STJ97710 200 µl
EUR 236.4
Description: Mouse monoclonal to IL-8.
Anti-IL-8 antibody
STJ97717 200 µl
EUR 236.4
Description: Mouse monoclonal to IL-8.
Anti-IL-8 antibody
STJ97720 200 µl
EUR 236.4
Description: Mouse monoclonal to IL-8.
1730 8 SNAP-SEAL 8 OZ
1730-8 100/pk
EUR 88.8
Description: Disposable Plastic; Plastic Containers
mAb mouse anti-human IL-8
CT264 0.5 mg
EUR 312
IL-8
E5-00004 1mg
EUR 1152
Anti-IL-8 Monoclonal Antibody
A00423-1 100ul
EUR 476.4
Description: Mouse Monoclonal Antibody for IL-8 Antibody (CXCL8) detection. Tested with IHC in Human, Mouse, Rat.
Individual Reaction Mix 8
G065-8 200 reactions
EUR 200.4
Swine IL-6 Recombinant Protein
R00102-8 5ug/vial
EUR 310.8
Description: Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. Swine IL-6 Recombinant Protein is purified interleukin-6 produced in yeast.
Swine IL-4 Recombinant Protein
R00230-8 5ug/vial
EUR 310.8
Description: IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. Swine IL-4 Recombinant Protein is purified interleukin-4 produced in yeast.
Rabbit IL-2 Recombinant Protein
R00387-8 5ug/vial
EUR 310.8
Description: Interleukin-2 (IL-2) is a cytokine produced by T-helper cells in response to antigenic or mitogenic stimulation. It is required for T-cell proliferation and other activities crucial to the regulation of the immune response. Rabbit IL-2 Recombinant Protein is purified interleukin-2 produced in yeast.
Swine IL-17A Recombinant Protein
R00421-8 5ug/vial
EUR 310.8
Description: IL-17A is a member of the IL-17 family of cytokines, whose members are involved in numerous immune regulatory functions. IL-17 induces the production of many other cytokines, chemokines, and prostaglandins. Swine IL-17A Recombinant Protein is purified interleukin-17A produced in yeast.
Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 418.8
Human Interleukin-8 (IL-8) Antibody
13102-05011 150 ug
EUR 248.4
Human IL-8 Antibody
33486-05111 150 ug
EUR 313.2
Human IL-8 Protein
abx060803-25ug 25 ug
EUR 644.4
Human IL-8 Protein
abx060804-25ug 25 ug
EUR 644.4
Recombinant Human IL-8
P0180 100ug
EUR 626.83
Description: Recombinant Human protein for IL-8
IL-8/CXCL8, Human
HY-P7224 50ug
EUR 596.4
Recombinant Human IL-8
SJB05-01 25µg/vial
EUR 342
Ovine IL-1 beta Recombinant Protein
R00101-8 5ug/vial
EUR 310.8
Description: IL-1 beta (IL-1β) is a member of the interleukin 1 family of cytokines. The IL-1 beta cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. Ovine IL-1 beta Recombinant Protein is purified interleukin-1 beta cytokine produced in yeast.
Human THSD7a ELISA kit
8-E01A2155
  • EUR 1524.00
  • EUR 624.00
  • EUR 822.00
  • 192 tests
  • 1 plate of 48 wells
  • 1 plate of 96 wells
Description: A competitive ELISA for quantitative measurement of Human THSD7a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Anti-bovine IL-8 (CXCL8) antibody
STJ15100008 250 µg
EUR 403.2
Description: These monoclonal antibodies enable sensitive and specific detection of bovine IL-8 in ELISpot.
Anti-bovine IL-8 (CXCL8) antibody
STJ15100009 250 µg
EUR 403.2
Description: This monoclonal antibody enables sensitive and specific detection of bovine IL-8 in ELISA.
IL-8, Interleukin-8, monkey
RC222-19 5ug
EUR 125.26
IL-8 Antibody
5149-100
EUR 405.6
IL-8 Inhibitor
5-01378 4 x 5mg Ask for price
IL-8 Antibody
BF0238 200ul
EUR 540
CXCL8/IL-8
E21-C97 10ug
EUR 411.6
CXCL8/IL-8
GT15156 100 ug
EUR 631.2
IL-8 Inhibitor
H-2268.0001 1.0mg
EUR 200.4
Description: Sum Formula: C45H66N18O7S; CAS# [138559-60-1] net
IL-8 Inhibitor
H-2268.0005 5.0mg
EUR 691.2
Description: Sum Formula: C45H66N18O7S; CAS# [138559-60-1] net
IL-8 Inhibitor
H-2268.0025 25.0mg
EUR 2648.4
Description: Sum Formula: C45H66N18O7S; CAS# [138559-60-1] net
Bovine(IL-8)
QY-E60063 96T
EUR 511.2
IL-8 Antibody
R30420 100 ug
EUR 419
IL-8 Antibody
R31932 100 ug
EUR 419
IL-8 Antibody
F51235-0.08ML 0.08 ml
EUR 165
IL-8 Antibody
F51235-0.4ML 0.4 ml
EUR 379
Human Interleukin 8 (IL-8) CLIA Kit
abx195916-96tests 96 tests
EUR 990
Human Interleukin 8,IL-8 ELISA KIT
201-12-0090 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
Human Interleukin 8, IL-8 ELISA KIT
CSB-E04641h-24T 1 plate of 24 wells
EUR 198
Description: Quantitative sandwich ELISA kit for measuring Human Interleukin 8, IL-8 in samples from serum, cell culture supernates, saliva, urine, cerebrospinalfluid (CSF), tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human Interleukin 8, IL-8 ELISA KIT
1-CSB-E04641h
  • EUR 600.00
  • EUR 4082.40
  • EUR 2184.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Human Interleukin 8, IL-8 in samples from serum, cell culture supernates, saliva, urine, cerebrospinalfluid(CSF), tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Human IL-8(Interleukin 8) ELISA Kit
EH0205 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml
Human Interleukin-8 (IL-8) ELISA Kit
LF-EK60006 1×96T
EUR 948
Human Interleukin 8(IL-8)ELISA Kit
GA-E0129HM-48T 48T
EUR 346.8
Human Interleukin 8(IL-8)ELISA Kit
GA-E0129HM-96T 96T
EUR 559.2
Human Interleukin 8(IL-8)ELISA Kit
QY-E04259 96T
EUR 433.2
Human IL-8 ELISA kit
CT212A 5-plate
EUR 554.4
Human IL-8 ELISA Kit
EHI0063 96Tests
EUR 625.2
human IL-8 His tag
E410A07-100 100μg
EUR 1152
IL-8 ELISA KIT|Human
EF003990 96 Tests
EUR 826.8
Human IL-8 ELISA kit
LF-EK50165 1×96T
EUR 777.6
rec Monocyte IL-8 (human)
H-9625.0005 5.0µg
EUR 194.4
Description: Sum Formula: C372H600N106O106S4; CAS# [142298-00-8]
rec Monocyte IL-8 (human)
H-9625.0025 25.0µg
EUR 457.2
Description: Sum Formula: C372H600N106O106S4; CAS# [142298-00-8]
IL-8/CXCL8, 72a.a., Human
HY-P7380 50ug
EUR 596.4
rec Endothelial IL-8 (human)
H-3742.0010 10.0µg
EUR 340.8
Description: Sum Formula: C397H644N114O111S4; CAS# [142298-01-9]
rec Endothelial IL-8 (human)
H-3742.0050 50.0µg
EUR 1167.6
Description: Sum Formula: C397H644N114O111S4; CAS# [142298-01-9]
IL-8 (Human) ELISA Kit
K4169-100
EUR 874.8
Recombinant Human IL-8 Protein
RP00052 5 μg
EUR 178.8
Bovine Cytokines IL-8(Cytokines IL-8) ELISA Kit
QY-E60102 96T
EUR 511.2
Pesticide Mix 8 Containing 15 Compounds in Acetonitrile
SPXPR-8 1ML
EUR 249.66
8 mm Crimp head ; Level 3 High Performance Applications
ECMJ-8 EACH
EUR 295.26
IL-8, CXCL8, Interleukin-8, canine
RC242-19 5ug
EUR 125.26
IL-8, CXCL8, Interleukin-8, porcine
RC282-19 5ug
EUR 125.26
Horse Interleukin 8 (IL-8) ELISA
QY-E120050 96T
EUR 573.6
Human Interleukin-8 (IL-8) Antibody (Biotin Conjugate)
13102-05021 150 ug
EUR 313.2
Recombinant Human Interleukin-8/IL-8 (C-6His)
CC97-10ug 10ug
EUR 175.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Recombinant Human Interleukin-8/IL-8 (C-6His)
CC97-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Recombinant Human Interleukin-8/IL-8 (C-6His)
CC97-500ug 500ug
EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Recombinant Human Interleukin-8/IL-8 (C-6His)
CC97-50ug 50ug
EUR 406.8
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Recombinant Human Interleukin-8/IL-8 (Ser28-Ser99)
C035-10ug 10ug
EUR 181.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Recombinant Human Interleukin-8/IL-8 (Ser28-Ser99)
C035-1mg 1mg
EUR 2203.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Recombinant Human Interleukin-8/IL-8 (Ser28-Ser99)
C035-500ug 500ug
EUR 1557.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Recombinant Human Interleukin-8/IL-8 (Ser28-Ser99)
C035-50ug 50ug
EUR 424.8
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Recombinant Human Interleukin-8/IL-8 (Ala23-Ser99)
C037-10ug 10ug
EUR 181.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Recombinant Human Interleukin-8/IL-8 (Ala23-Ser99)
C037-1mg 1mg
EUR 2203.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Recombinant Human Interleukin-8/IL-8 (Ala23-Ser99)
C037-500ug 500ug
EUR 1557.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Recombinant Human Interleukin-8/IL-8 (Ala23-Ser99)
C037-50ug 50ug
EUR 412.8
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
CLIA kit for Human IL-8 (Interleukin 8)
E-CL-H0048 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Human IL-8 (Interleukin 8) in samples from Serum, Plasma, Cell supernatant
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