IPREM/LCABIE Laboratoire de Chimie Analytique Bio-inorganique et Environnement

Pathogenic bacteria and virusses

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Modified Vaccinia Virus Ankara as a Viral Vector for Vaccine Candidates against Chikungunya Virus

There’s a must develop a extremely efficient vaccine in opposition to the rising chikungunya virus (CHIKV), a mosquito-borne Alphavirus that causes extreme illness in people consisting of acute febrile sickness, adopted by persistent debilitating polyarthralgia and polyarthritis.
On this assessment, we offer a short historical past of the event of the primary poxvirus vaccines that led to smallpox eradication and its implications for additional vaccine improvement. For instance, we summarize the event of vaccine candidates primarily based on the modified vaccinia virus Ankara (MVA) vector expressing totally different CHIKV structural proteins, paying particular consideration to MVA-CHIKV expressing the entire CHIKV structural proteins: C, E3, E2, 6K and E1.
We assessment the characterization of innate and adaptive immune responses induced in mice and nonhuman primates by the MVA-CHIKV vaccine candidate and study its efficacy in animal fashions, with promising preclinical findings wanted previous to the approval of human scientific trials.

The Challenges of Vaccine Growth in opposition to Betacoronaviruses: Antibody Dependent Enhancement and Sendai Virus as a Potential Vaccine Vector

 

To design an efficient and protected vaccine in opposition to betacoronaviruses, it’s vital to make use of their evolutionarily conservative antigenic determinants that may elicit the mix of robust humoral and cell-mediated immune responses. Concentrating on such determinants minimizes the chance of antibody-dependent enhancement of viral an infection. This phenomenon was noticed in animal trials of experimental vaccines in opposition to SARS-CoV-1 and MERS-CoV that had been developed primarily based on inactivated coronavirus or vector constructs expressing the spike protein (S) of the virion.

The substitution and glycosylation of sure amino acids within the antigenic determinants of the S-protein, in addition to its conformational modifications, can result in the identical impact in a brand new experimental vaccine in opposition to SARS-CoV-2. Utilizing extra conservative structural and accent viral proteins for the vaccine antigenic determinants will assist to keep away from this drawback. This assessment outlines approaches for growing vaccines in opposition to the brand new SARS-CoV-2 coronavirus which might be primarily based on non-pathogenic viral vectors.

For environment friendly prevention of infections brought on by respiratory pathogens the flexibility of the vaccine to stimulate mucosal immunity within the respiratory tract is necessary. Such a vaccine will be developed utilizing non-pathogenic Sendai virus vector, since it may be administered intranasally and induce a mucosal immune response that strengthens the antiviral barrier within the respiratory tract and gives dependable safety in opposition to an infection.

 

Growth of Excessive Decision DNA Melting Evaluation for Simultaneous Detection of Potato Mop-High Virus and Its Vector, Spongospora subterranea, in Soil

 

On this examine, a set of duplex reverse transcription (RT)-PCR-mediated excessive decision DNA melting (HRM) analyses for simultaneous detection of potato mop-virus (PMTV) and its protist vector, Spongospora subterranea f.sp. subterranea (Sss), was developed.

The infestation of soil by PMTV was detected by utilizing a tobacco-based baiting system. Complete RNA extracted from the soil led to profitable RT-PCR gel-electrophoresis detection of each PMTV and Sss. To facilitate extra environment friendly detection, newly designed primer pairs for PMTV RNA species (i.e., RNA-Rep, -CP, and -TGB) had been analyzed along with the present Sss primers utilizing real-time RT-PCR.

The ensuing amplicons exhibited melting profiles that might be readily differentiated. Beneath duplex RT-PCR format, all PMTV and Sss primer mixtures led to profitable detection of respective PMTV RNA species and Sss within the samples by excessive decision DNA melting (HRM) analyses. When the duplex HRM assay was utilized to soil samples collected from six fields at 4 totally different websites in New Brunswick, Canada, optimistic detection of PMTV and/or Sss was present in 63-100% samples collected from fields by which PMTV-infected tubers had been noticed.

In distinction, the samples from fields the place neither PMTV- nor Sss-infected tubers had been noticed resulted in unfavorable detection by the assay. Bait tobacco bioassay for PMTV and Sss produced comparable outcomes. Between 63%-83% and 100% of the soil samples collected from PMTV-infested fields led to PMTV and Sss infections within the bait tobacco crops, respectively; whereas no PMTV or Sss contaminated crops had been obtained from soil samples collected from PMTV/Sss-free fields.

Accelerating the Morphogenetic Cycle of the Viral Vector Aedes aegypti Larvae for Quicker Larvicidal Bioassays

 

Any bioassay to check new chemically synthesized larvicides or phytolarvicides in opposition to Culicidae and extra dangerous mosquito species, similar to Aedes aegypti and Aedes albopictus, which particularly transmit dengue, yellow fever, chikungunya viral fevers in addition to Zika virus, or Anopheles gambiae, a vector for malaria and philariasis, requires hundreds of well-developed larvae, ideally on the fourth instar stage. The pure morphogenetic cycle of Aedes spp., within the subject or within the laboratory, could lengthen to 19 days at room temperature (e.g., 25°C) from the first everlasting contact between viable eggs and water and the final stage of larval development or metamorphosis into flying adults. Thus, accelerated sequential molting is fascinating for swifter bioassays of larvicides.

We achieved this purpose in Aedes aegypti with very restricted strategic and low-cost additions to meals, similar to coconut water, milk or its casein, yeast extract, and to a lesser extent, glycerol. The naturally wealthy coconut water was glorious for shortly attaining the inhabitants of instar IV larvae, probably the most superior one earlier than pupation, saving a couple of week, for subsequent larvicidal bioassays. Diluted milk, as one other meals supply, allowed a fair sooner ultimate ecdysis and adults are helpful for mosquito taxonomical goal.

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Security and Tolerability of the Adeno-Related Virus Vector, AAV6.2FF, Expressing a Monoclonal Antibody in Murine and Ovine Animal Fashions

 

Adeno-associated virus (AAV) vector mediated expression of therapeutic monoclonal antibodies is another technique to conventional vaccination to generate immunity in immunosuppressed or immunosenescent people. On this examine, we vectorized a human monoclonal antibody (31C2) directed in opposition to the spike protein of SARS-CoV-2 and decided the protection profile of this AAV vector in mice and sheep as a big animal mannequin. In each research, plasma biochemical parameters and hematology had been similar to untreated controls.
Aside from gentle myositis on the web site of injection, not one of the main organs revealed any indicators of toxicity. AAV-mediated human IgG expression elevated steadily all through the 28-day examine in sheep, leading to peak concentrations of 21.4-46.7 µg/ mL, demonstrating sensible scale up from rodent to giant animal fashions. This different strategy to immunity is price additional exploration after this demonstration of security, tolerability, and scalability in a big animal mannequin.

IL-6, Interleukin-6, human
RC212-17 5ug
EUR 125.26

IL-17, Human
HY-P7036 10ug
EUR 253.2

IL-17
MO15110 500 ug
EUR 1092

IL-17
EF014060 96 Tests
EUR 826.8

IL-17
E5-00007 1mg
EUR 1152

IL-17
PR27113 5 ug
EUR 229.2

Anti-IL-17 alpha antibody
STJ93683 200 µl
EUR 236.4
Description: Rabbit polyclonal to IL-17Ralpha.

IL-17/IL-17A
E21-021 10ug
EUR 411.6

Recombinant Human IL-17
SJB09-03 25µg/vial
EUR 307.2

IL-6, Interleukin-6, rat
RC252-17 2ug
EUR 125.26

Interleukin 17 (IL-17) Antibody
abx234224-100ug 100 ug
EUR 610.8

Interleukin 17 (IL-17) Antibody
abx234225-100ug 100 ug
EUR 577.2

Bovine Interleukin 17(IL-17)
QY-E60069 96T
EUR 511.2

Human Interleukin 17 (IL-17) CLIA Kit
abx195860-96tests 96 tests
EUR 990

Human Interleukin 17,IL-17 ELISA Kit
201-12-0143 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Interleukin 17,IL-17 ELISA Kit
CN-03335H1 96T
EUR 520.8

Human Interleukin 17,IL-17 ELISA Kit
CN-03335H2 48T
EUR 340.8

Human Interleukin 17(IL-17)ELISA Kit
GA-E0081HM-48T 48T
EUR 346.8

Human Interleukin 17(IL-17)ELISA Kit
GA-E0081HM-96T 96T
EUR 559.2

IL-17 Interleukin-17 Human Recombinant Protein
PROTQ16552-1 Regular: 25ug
EUR 380.4
Description: Interleukin-17A Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 264 amino acids (2 chains of 132 aa) and having a molecular mass of 31kDa.  ;The IL-17 is purified by proprietary chromatographic techniques.

Human Interleukin 17(IL-17)ELISA Kit
QY-E04323 96T
EUR 433.2

Human Interleukin 17,IL-17 ELISA Kit
YLA1538HU-48T 48T
EUR 435

Human Interleukin 17,IL-17 ELISA Kit
YLA1538HU-96T 96T
EUR 562.5

IL-17 Antibody
4877-002mg 0.02 mg
EUR 206.18
Description: IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.

IL-17 Antibody
4877-01mg 0.1 mg
EUR 523.7
Description: IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.

IL-17 Peptide
4877P 0.05 mg
EUR 197.7
Description: (NT) IL-17 peptide

IL-17 Antibody
4887-002mg 0.02 mg
EUR 206.18
Description: IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.

IL-17 Antibody
4887-01mg 0.1 mg
EUR 523.7
Description: IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.

IL-17 Peptide
4887P 0.05 mg
EUR 197.7
Description: (IN) IL-17 peptide

IL-17 Antibody
24784-100ul 100ul
EUR 468

IL-17 Antibody
24789-100ul 100ul
EUR 468

IL-17 Antibody
R35097-100UG 100 ug
EUR 399

IL-17 Antibody
R36188-100UG 100 ug
EUR 399

IL-17 Antibody
F42568-0.4ML 0.4 ml
EUR 379

IL-17 Antibody
5176-100 each
EUR 405.6

IL-6, Interleukin-6, murine (mouse)
RC232-17 2ug
EUR 125.26

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 418.8

Recombinant Human IL-17 (IL-17A) Protein
PROTQ16552-4 25ug
EUR 380.4
Description: The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules.  Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles.  The six known members of this family, IL-17A through IL-17F, are secreted as homodimers.  IL-17A exhibits cross-species bioactivity between human and murine cells.  Recombinant human IL-17A is a 31.3 kDa disulfide-linked homodimer of two 137 amino acid polypeptide chains.

IL-6, Interleukin-6, monkey (rhesus macaque)
RC222-17 2ug
EUR 125.26

IL-17 ELISA KIT|Human
EF006939 96 Tests
EUR 826.8

human IL-17, His tag
E410A10-100 100μg
EUR 1152

Human IL-17 ELISA kit
LF-EK50201 1×96T
EUR 777.6

IL-17 (Human) ELISA Kit
E4628-100 each
EUR 874.8

Human Interleukin 17 (IL-17) Detection Assay Kit
6808 1 kit
EUR 580.26
Description: Human Interleukin 17 (IL-17) Detection Assay Kit

CLIA kit for Human IL-17 (Interleukin 17)
E-CL-H0104 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Human IL-17 (Interleukin 17) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human IL-17 (Interleukin 17)
E-EL-H0105 1 plate of 96 wells
EUR 452.4
Description: A sandwich ELISA kit for quantitative measurement of Human IL-17 (Interleukin 17) in samples from Serum, Plasma, Cell supernatant

IL-17 Interleukin-17 Human Recombinant Protein, HEK
PROTQ16552 Regular: 10ug
EUR 380.4
Description: IL-17 Human Recombinant produced in HEK cells is a glycosylated homodimer, having a molecular weight range of 30-35kDa due to glycosylation.;The IL-17 is purified by proprietary chromatographic techniques.

17-Hydroxyprogesterone (17-OHP) ELISA Kit
DLR-17-OHP-Ge-48T 48T
EUR 562.8
Description: A competitive inhibition quantitative ELISA assay kit for detection of 17-Hydroxyprogesterone (17-OHP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

17-Hydroxyprogesterone (17-OHP) ELISA Kit
DLR-17-OHP-Ge-96T 96T
EUR 729.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of 17-Hydroxyprogesterone (17-OHP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

IL-17 Recombinant Protein
91-015 0.05 mg
EUR 437.1
Description: Interleukin-17 is a potent pro-inflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. As IL-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. IL-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation.

IL-17 Recombinant Protein
91-616 0.05 mg
EUR 588.3
Description: Interleukin-17 is a potent pro-inflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. As IL-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. IL-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation.

Polyclonal IL-17 Antibody
APR06508G 0.1 mg
EUR 790.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IL-17 . This antibody is tested and proven to work in the following applications:

Polyclonal IL-17 Antibody
APR06512G 0.1 mg
EUR 790.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IL-17 . This antibody is tested and proven to work in the following applications:

rHu IL-17-A
AK8249-0005 5µg Ask for price

rHu IL-17-A
AK8249-0025 25µg Ask for price

rHu IL-17-A
AK8249-0100 100µg Ask for price

rHu IL-17-A
AK8249-1000 1mg Ask for price

rHu IL-17-E
AK8299-0005 5µg Ask for price

rHu IL-17-E
AK8299-0025 25µg Ask for price

rHu IL-17-E
AK8299-0100 100µg Ask for price

rHu IL-17-E
AK8299-1000 1mg Ask for price

rHu IL-17-F
AK8312-0005 5µg Ask for price

rHu IL-17-F
AK8312-0025 25µg Ask for price

rHu IL-17-F
AK8312-0100 100µg Ask for price

rHu IL-17-F
AK8312-1000 1mg Ask for price

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit
RD-17-OHP-Ge-48Tests 48 Tests
EUR 560.4

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit
RD-17-OHP-Ge-96Tests 96 Tests
EUR 775.2

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit
RDR-17-OHP-Ge-48Tests 48 Tests
EUR 585.6

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit
RDR-17-OHP-Ge-96Tests 96 Tests
EUR 811.2

Mouse Interleukin 17 (IL-17) CLIA Kit
abx195861-96tests 96 tests
EUR 990

Rat Interleukin 17 (IL-17) CLIA Kit
abx195862-96tests 96 tests
EUR 990

Rabbit Interleukin 17,IL-17 ELISA Kit
CN-00640R1 96T
EUR 542.4

Rabbit Interleukin 17,IL-17 ELISA Kit
CN-00640R2 48T
EUR 362.4

Chicken Interleukin 17,IL-17 ELISA Kit
CN-00838C1 96T
EUR 542.4

Chicken Interleukin 17,IL-17 ELISA Kit
CN-00838C2 48T
EUR 362.4

PoFAine interleukin 17,IL-17 ELISA Kit
CN-01210P2 48T
EUR 391.2

PoFAine interleukin 17,IL-17 ELISA Kit
CN-01211P1 96T
EUR 538.8

PoFAine interleukin 17,IL-17 ELISA Kit
CN-01211P2 48T
EUR 358.8

Rat Interleukin 17,IL-17 ELISA KIT
CN-01495R1 96T
EUR 538.8

Rat Interleukin 17,IL-17 ELISA KIT
CN-01495R2 48T
EUR 358.8

Canine Interleukin 17(IL-17)ELISA Kit
CSB-E17935c-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Canine Interleukin 17 (IL-17) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Canine Interleukin 17(IL-17)ELISA Kit
1-CSB-E17935c
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Canine Interleukin 17(IL-17) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Interleukin 17, IL-17 ELISA KIT
CSB-E07451r-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat Interleukin 17, IL-17 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Interleukin 17, IL-17 ELISA KIT
1-CSB-E07451r
  • EUR 723.60
  • EUR 5096.40
  • EUR 2712.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Interleukin 17, IL-17 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Pig interleukin 17, IL-17 ELISA Kit
CSB-E08057p-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Pig interleukin 17, IL-17 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Pig interleukin 17, IL-17 ELISA Kit
1-CSB-E08057p
  • EUR 723.60
  • EUR 5096.40
  • EUR 2712.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Pig interleukin 17, IL-17 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rabbit Interleukin 17, IL-17 ELISA Kit
CSB-E08058Rb-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rabbit Interleukin 17, IL-17 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rabbit Interleukin 17, IL-17 ELISA Kit
1-CSB-E08058Rb
  • EUR 723.60
  • EUR 5096.40
  • EUR 2712.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rabbit Interleukin 17, IL-17 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
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