lcabie

Potential of chitosan/alginate nanoparticles as a non-viral vector for gene delivery: Formulation and optimization using D-optimal design

Chitosan/alginate (Chi/Alg) nanoparticles as a non-viral vector for the Smad4 encoding plasmid have been optimized using D-optimal design primarily based on the nanoparticles/plasmid ratio, Chi/Alg MW, and preparation technique sort. Following the optimization and validation of the most effective formulation, morphology research and FTIR measurements have been carried out to guage the optimized Chi/Alg/S NPs. Toxicity (MTT assay) and transfection research have been carried out for the most effective formulation as compared with Lipofectamine 2000, and Polyethyleneimine (PEI) and evaluated utilizing Inexperienced Fluorescence Protein (GFP) assay, Move cytometry, and RT-PCR.
The mannequin predicted a particle measurement of 111 nm, loading efficacy (LE) of 43%, cumulative launch (CMR) of 39%, the ζ-potential of +50 mV, and PDI of 0.13. The anticipated level situation was as follows: NP ratio = 13, Chi/Alg MW ratio = 2.35, and preparation technique sort = 1. Microscopic findings revealed that the form of nanoparticles was spherical. The Chi/Alg/S nanoparticles confirmed no toxicity and transfection efficacy of 29.9% was noticed as compared with Lipofectamine (35.5%) and PEI (30.9%).

Spatial distribution and habitat choice of culicoides imicola: The potential vector of bluetongue virus in Tunisia

The rising menace of vector-borne illnesses (VBDs) represents an awesome problem to those that handle public and animal well being. Figuring out the spatial distribution of arthropod vector species is an important step in learning the danger of transmission of a vector-borne pathogen (VBP) and in estimating threat ranges of VBD. Threat maps enable higher focusing on surveillance and assist in designing management measures.
We aimed to review the geographical distribution of Culicoides imicola, the primary competent vector of Bluetongue virus (BTV) in sheep in Tunisia. Fifty-three information overlaying the entire distribution vary of C.imicola in Tunisia have been obtained throughout a 2-year subject entomological survey (August 2017 – January 2018 and August 2018 – January 2019).
The ecological area of interest of C. imicola is described utilizing ecological-niche issue evaluation (ENFA) and Mahalanobis distances issue evaluation (MADIFA). An environmental suitability map (ESM) was developed by MaxEnt software program to map the optimum habitat below the present local weather background. The MaxEnt mannequin was extremely correct with a statistically vital space below curve (AUC) worth of 0.941. The situation of the potential distribution of C. imicola is predicted in specified areas of Tunisia. Our findings might be utilized in varied methods akin to surveillance and management program of BTV in Tunisia.
lcabie
lcabie

Analysis of a brand new viral vaccine vector in mice and rhesus macaques: J paramyxovirus (JPV)-expressing HA of influenza A virus H5N1

H5N1, an avian influenza virus, is thought to flow into in lots of Asian nations like Bangladesh, China, Cambodia, Indonesia, and Vietnam. The present FDA-approved H5N1 vaccine has a reasonable degree of efficacy. A protected and efficient vaccine is required to stop the outbreaks of extremely pathogenic avian influenza (HPAI) H5N1 in people. Non-segmented negative-sense single-stranded viruses (NNSVs) are broadly used as a vector to develop vaccines for people, animals, and poultry. NNSVs stably categorical international genes with out integrating with the host genome. J Paramyxovirus (JPV) is a non-segmented negative-strand RNA virus and a member of the proposed genus Jeilongvirus within the household Paramyxoviridae. JPV-specific antibodies have been detected in rodents, bats, people, and pigs, however the virus just isn’t related to illness in any species apart from mice. JPV replicates within the respiratory tract of mice and effectively expresses the virus-vectored international genes in tissue tradition cells. On this work, we explored JPV as a vector for creating an H5N1 vaccine utilizing intranasal supply. We integrated hemagglutinin (HA) of H5N1 into the JPV genome by changing the small hydrophobic (SH) gene to generate a recombinant JPV expressing HA (rJPV-ΔSH-H5). A single intranasal administration of rJPV-ΔSH-H5 protected mice from a deadly HPAI H5N1 problem. Intranasal vaccination of rJPV-ΔSH-H5 in rhesus macaques elicited antigen-specific humoral and cell-mediated immune responses. This work demonstrates that JPV is a promising vaccine vector. IMPORTANCE HPAI H5N1 outbreak in Southeast Asia destroyed hundreds of thousands of birds. Transmission of H5N1 into people resulted in deaths in lots of nations. On this work, we developed a novel H5N1 vaccine candidate utilizing JPV as a vector and demonstrated that JPV is an efficacious vaccine vector in animals. NNSVs stably categorical international genes with out integrating into the host genome. JPV, an NNSV, replicates effectively within the respiratory tract and induces strong immune responses.

Agro-waste derived compounds (flax and black seed peels): Toxicological impact towards the West Nile virus vectorCulex pipiens L. with particular reference to GC-MS evaluation

The event of various approaches to make use of agricultural residues as a supply of excessive value-added merchandise, develop into a should, particularly after the issues emerged as a consequence of their accumulation. This contribution demonstrates the potential of agricultural residues, Linuim usitatissium (flax seed) and Nigella sativa (black seed) peels, as uncooked supplies for the manufacturing of bioactive merchandise, botanical pesticides, towards Cx. pipiens, with deep evaluation to their chemical constituents by gasoline chromatography-mass spectrometry, the larvicidal efficacies of the three crude extracts (methylene chloride, petroleum ether and methanol 70%) from the 2 plant waste peels have been evaluated for the primary time towards the late third instar larvae of Cx. pipiens. Outcomes indicated completely different deadly doses in larvae relying on the efficacy of natural solvent used.
For each compounds methanol 70% extracts produced the best dry yield. Essentially the most environment friendly solvent is petroleum ether in case of each flax and Black seed peels. Petroleum ether extract exhibited the best toxicity towards Cx. pipiens with an LC50 of 69.6383 ppm. The identical outcomes for black seed indicated that petroleum ether was essentially the most environment friendly towards Cx. pipiens with an LC50 of 40.7748 ppm. The examine revealed for the primary time the kind of phytochemical constituents presents in peels of flax and black seeds utilizing GC-MS evaluation which revealed twenty-eight constituents amongst extracts of flax and black seed peels starting from to 58.8711% to 99.99% of the whole extracts. GC-MS profiling confirmed {that a} 5 constituents, 9-2-Methyl-Z, Z-3, 13 octadecadienol (terpenoid), 9,17-Octadecadienal, (Z)-, Nonanoic acid, 9-oxo-, methyl ester, 9,12-Octadecadienoic acid Z,Z and Octasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11,13,13,15,15-hexadecamethyl- have insecticidal exercise beside many different organic actions as recorded from quite a lot of botanical extracts. Whereas the constituents like Hexadecanoic acid, methyl ester and cis-9-Hexadecenal, each of them are larvicidal, cis-Vaccenic acid and 9-Oxononanoic acid exhibiting solely an insecticidal exercise beside Undecanoic acid the mosquito repellent. The opposite six constituents Linoelaidic acid, Oleic Acid, Z-2-Octadecen-1-ol, 1-Methoxy-3-hydroxymethylheptane, Cis-11,14-Eicosadieonic acid-methyl ester and Heptasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11,13,13-tetradecamethyl- are constituents of different plant extracts which confirmed as a complete an insecticidal exercise.

Recombinant Mouse LIF Protein

R01400-1 100ug/vial
EUR 352.8

ELISA kit for Mouse LIF

EK5250 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse LIF PicoKine ELISA Kit

EK0580 96 wells
EUR 510
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA).

Lif ORF Vector (Mouse) (pORF)

ORF049145 1.0 ug DNA
EUR 607.2

Lif ORF Vector (Mouse) (pORF)

ORF049146 1.0 ug DNA
EUR 607.2

LIF, Mouse Recombinant , 1MIU/ml

L4500-001 1ml
EUR 406.8

LIF, Mouse Recombinant , 1MIU/ml

L4500-005 5x1ml
EUR 1431.6

LIF ELISA Kit (Mouse) (OKEH04045)

OKEH04045 96 Wells
EUR 652.8
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL

LIF ELISA Kit (Mouse) (OKBB00760)

OKBB00760 96 Wells
EUR 606
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg>

LIF, Mouse Recombinant , 10MIU/ml

L4501-001 1ml
EUR 1744.8

Nori® Mouse LIF ELISA Kit

GR117058 96-well
EUR 461

Human CellExp? LIF, mouse recombinant

7268-10 each
EUR 444

Leukemia Inhibitory Factor (LIF), mouse

PR16102 5 ug
EUR 522

Lif sgRNA CRISPR Lentivector set (Mouse)

K3872501 3 x 1.0 ug
EUR 406.8

LIF Antibody

6245-002mg 0.02 mg
EUR 206.18
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Antibody

6245-01mg 0.1 mg
EUR 523.7
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Antibody

5216-100 each
EUR 379.2

LIF Antibody

5216-30T each
EUR 175.2

LIF antibody

70R-12299 100 ug
EUR 483.6
Description: Rabbit polyclonal LIF antibody

LIF antibody

70R-14100 100 ug
EUR 386.4
Description: Affinity purified Rabbit polyclonal LIF antibody

LIF Antibody

25283-100ul 100ul
EUR 468

LIF antibody

70R-51295 100 ul
EUR 292.8
Description: Purified Polyclonal LIF antibody

LIF antibody

70R-6230 50 ug
EUR 560.4
Description: Rabbit polyclonal LIF antibody

LIF Antibody

36950-100ul 100ul
EUR 302.4

LIF Antibody

1-CSB-PA012928ESR2HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA

LIF Antibody

1-CSB-PA198481
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100

LIF Antibody

1-CSB-PA004761
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

LIF Antibody

DF13730 100ul
EUR 420

LIF Antibody

F49998-0.08ML 0.08 ml
EUR 140.25
Description: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

LIF Antibody

F49998-0.4ML 0.4 ml
EUR 322.15
Description: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

LIF antibody

PAab09804 100 ug
EUR 463.2

LIF Antibody

R36251-100UG 100 ug
EUR 339.15
Description: Additional name(s) for this target protein: Leukemia inhibitory factor

LIF Antibody

R30639 100 ug
EUR 356.15
Description: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. It is produced in high amounts by the human endometrium and the trophoblast itself, and its receptors are present on cytotrophoblast cells. LIF could thus play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface. The gene maps to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2.

LIF Antibody

R31467 100 ug
EUR 356.15
Description: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. It could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse)

4-PAA085Mu01
  • EUR 283.20
  • EUR 2805.60
  • EUR 703.20
  • EUR 352.80
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF)

LIF, Leukemia inhibitory factor, murine (mouse)

RC234-18 5ug
EUR 125.26

Mouse Leukemia Inhibitory Factor (LIF) Protein

20-abx067761
  • EUR 794.40
  • EUR 326.40
  • EUR 2414.40
  • EUR 944.40
  • EUR 577.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Leukemia Inhibitory Factor (LIF) Protein

abx262959-1mg 1 mg
EUR 3801.6

Mouse Leukemia Inhibitory Factor (LIF) Protein

abx262959-3x10g 3x10 µg
EUR 393.6

Mouse Leukemia Inhibitory Factor (LIF) Protein

abx262959-3x2g 3x2 µg
EUR 276

anti-LIF

YF-PA12952 50 ug
EUR 435.6
Description: Mouse polyclonal to LIF

LIF Protein Vector (Mouse) (pPM-C-HA)

PV196580 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPM-C-HA)

PV196584 500 ng
EUR 723.6

Recombinant Mouse Leukemia Inhibitory Factor/lIF

C690-10ug 10ug
EUR 175.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Mouse Leukemia Inhibitory Factor/lIF

C690-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Mouse Leukemia Inhibitory Factor/lIF

C690-500ug 500ug
EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Mouse Leukemia Inhibitory Factor/lIF

C690-50ug 50ug
EUR 363.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

LIF Protein Vector (Mouse) (pPB-C-His)

PV196578 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPB-N-His)

PV196579 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPM-C-His)

PV196581 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPB-C-His)

PV196582 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPB-N-His)

PV196583 500 ng
EUR 723.6

LIF Protein Vector (Mouse) (pPM-C-His)

PV196585 500 ng
EUR 723.6

Mouse Lif(Leukemia inhibitory factor) ELISA Kit

EM0237 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 46.9pg/ml

Mouse Lif/ Leukemia inhibitory factor ELISA Kit

E1656Mo 1 Kit
EUR 655.2

Mouse LIF Protein (Ser 24-Phe 203) [His]

VAng-1946Lsx-20g 20 µg
EUR 588
Description: Mouse LIF protein, His tag, expressed in human 293 cells. (Uniprot ID: P09056)

Mouse LIF Protein (Ser 24-Phe 203) [His]

VAng-1946Lsx-50g 50 µg
EUR 1050
Description: Mouse LIF protein, His tag, expressed in human 293 cells. (Uniprot ID: P09056)

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), PE

4-PAA085Mu01-PE
  • EUR 339.60
  • EUR 2942.40
  • EUR 843.60
  • EUR 423.60
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with PE.

Recombinant Mouse LIF Protein, Untagged, E.coli-1mg

QP4235-1mg 1mg
EUR 3189.6

Recombinant Mouse LIF Protein, Untagged, E.coli-5ug

QP4235-5ug 5ug
EUR 163.2

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), APC

4-PAA085Mu01-APC
  • EUR 394.80
  • EUR 3649.20
  • EUR 1024.80
  • EUR 499.20
  • EUR 254.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with APC.

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), Cy3

4-PAA085Mu01-Cy3
  • EUR 476.40
  • EUR 4815.60
  • EUR 1316.40
  • EUR 615.60
  • EUR 289.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with Cy3.

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), HRP

4-PAA085Mu01-HRP
  • EUR 362.40
  • EUR 3182.40
  • EUR 907.20
  • EUR 452.40
  • EUR 240.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with HRP.

Recombinant Mouse LIF Protein, Untagged, E.coli-20ug

QP4235-20ug 20ug
EUR 284.4

Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Mouse), FITC

4-PAA085Mu01-FITC
  • EUR 339.60
  • EUR 2942.40
  • EUR 843.60
  • EUR 423.60
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Leukemia Inhibitory Factor (LIF). This antibody is labeled with FITC.

Recombinant Mouse LIF Protein, Untagged, E.coli-100ug

QP4235-100ug 100ug
EUR 729.6