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Potential of chitosan/alginate nanoparticles as a non-viral vector for gene delivery: Formulation and optimization using D-optimal design
Chitosan/alginate (Chi/Alg) nanoparticles as a non-viral vector for the Smad4 encoding plasmid have been optimized using D-optimal design primarily based on the nanoparticles/plasmid ratio, Chi/Alg MW, and preparation technique sort. Following the optimization and validation of the most effective formulation, morphology research and FTIR measurements have been carried out to guage the optimized Chi/Alg/S NPs. Toxicity (MTT assay) and transfection research have been carried out for the most effective formulation as compared with Lipofectamine 2000, and Polyethyleneimine (PEI) and evaluated utilizing Inexperienced Fluorescence Protein (GFP) assay, Move cytometry, and RT-PCR.
The mannequin predicted a particle measurement of 111 nm, loading efficacy (LE) of 43%, cumulative launch (CMR) of 39%, the ζ-potential of +50 mV, and PDI of 0.13. The anticipated level situation was as follows: NP ratio = 13, Chi/Alg MW ratio = 2.35, and preparation technique sort = 1. Microscopic findings revealed that the form of nanoparticles was spherical. The Chi/Alg/S nanoparticles confirmed no toxicity and transfection efficacy of 29.9% was noticed as compared with Lipofectamine (35.5%) and PEI (30.9%).
Spatial distribution and habitat choice of culicoides imicola: The potential vector of bluetongue virus in Tunisia
The rising menace of vector-borne illnesses (VBDs) represents an awesome problem to those that handle public and animal well being. Figuring out the spatial distribution of arthropod vector species is an important step in learning the danger of transmission of a vector-borne pathogen (VBP) and in estimating threat ranges of VBD. Threat maps enable higher focusing on surveillance and assist in designing management measures.
We aimed to review the geographical distribution of Culicoides imicola, the primary competent vector of Bluetongue virus (BTV) in sheep in Tunisia. Fifty-three information overlaying the entire distribution vary of C.imicola in Tunisia have been obtained throughout a 2-year subject entomological survey (August 2017 – January 2018 and August 2018 – January 2019).
The ecological area of interest of C. imicola is described utilizing ecological-niche issue evaluation (ENFA) and Mahalanobis distances issue evaluation (MADIFA). An environmental suitability map (ESM) was developed by MaxEnt software program to map the optimum habitat below the present local weather background. The MaxEnt mannequin was extremely correct with a statistically vital space below curve (AUC) worth of 0.941. The situation of the potential distribution of C. imicola is predicted in specified areas of Tunisia. Our findings might be utilized in varied methods akin to surveillance and management program of BTV in Tunisia.
Analysis of a brand new viral vaccine vector in mice and rhesus macaques: J paramyxovirus (JPV)-expressing HA of influenza A virus H5N1
H5N1, an avian influenza virus, is thought to flow into in lots of Asian nations like Bangladesh, China, Cambodia, Indonesia, and Vietnam. The present FDA-approved H5N1 vaccine has a reasonable degree of efficacy. A protected and efficient vaccine is required to stop the outbreaks of extremely pathogenic avian influenza (HPAI) H5N1 in people. Non-segmented negative-sense single-stranded viruses (NNSVs) are broadly used as a vector to develop vaccines for people, animals, and poultry. NNSVs stably categorical international genes with out integrating with the host genome. J Paramyxovirus (JPV) is a non-segmented negative-strand RNA virus and a member of the proposed genus Jeilongvirus within the household Paramyxoviridae. JPV-specific antibodies have been detected in rodents, bats, people, and pigs, however the virus just isn’t related to illness in any species apart from mice. JPV replicates within the respiratory tract of mice and effectively expresses the virus-vectored international genes in tissue tradition cells. On this work, we explored JPV as a vector for creating an H5N1 vaccine utilizing intranasal supply. We integrated hemagglutinin (HA) of H5N1 into the JPV genome by changing the small hydrophobic (SH) gene to generate a recombinant JPV expressing HA (rJPV-ΔSH-H5). A single intranasal administration of rJPV-ΔSH-H5 protected mice from a deadly HPAI H5N1 problem. Intranasal vaccination of rJPV-ΔSH-H5 in rhesus macaques elicited antigen-specific humoral and cell-mediated immune responses. This work demonstrates that JPV is a promising vaccine vector. IMPORTANCE HPAI H5N1 outbreak in Southeast Asia destroyed hundreds of thousands of birds. Transmission of H5N1 into people resulted in deaths in lots of nations. On this work, we developed a novel H5N1 vaccine candidate utilizing JPV as a vector and demonstrated that JPV is an efficacious vaccine vector in animals. NNSVs stably categorical international genes with out integrating into the host genome. JPV, an NNSV, replicates effectively within the respiratory tract and induces strong immune responses.
Agro-waste derived compounds (flax and black seed peels): Toxicological impact towards the West Nile virus vector, Culex pipiens L. with particular reference to GC-MS evaluation
The event of various approaches to make use of agricultural residues as a supply of excessive value-added merchandise, develop into a should, particularly after the issues emerged as a consequence of their accumulation. This contribution demonstrates the potential of agricultural residues, Linuim usitatissium (flax seed) and Nigella sativa (black seed) peels, as uncooked supplies for the manufacturing of bioactive merchandise, botanical pesticides, towards Cx. pipiens, with deep evaluation to their chemical constituents by gasoline chromatography-mass spectrometry, the larvicidal efficacies of the three crude extracts (methylene chloride, petroleum ether and methanol 70%) from the 2 plant waste peels have been evaluated for the primary time towards the late third instar larvae of Cx. pipiens. Outcomes indicated completely different deadly doses in larvae relying on the efficacy of natural solvent used.
For each compounds methanol 70% extracts produced the best dry yield. Essentially the most environment friendly solvent is petroleum ether in case of each flax and Black seed peels. Petroleum ether extract exhibited the best toxicity towards Cx. pipiens with an LC50 of 69.6383 ppm. The identical outcomes for black seed indicated that petroleum ether was essentially the most environment friendly towards Cx. pipiens with an LC50 of 40.7748 ppm. The examine revealed for the primary time the kind of phytochemical constituents presents in peels of flax and black seeds utilizing GC-MS evaluation which revealed twenty-eight constituents amongst extracts of flax and black seed peels starting from to 58.8711% to 99.99% of the whole extracts. GC-MS profiling confirmed {that a} 5 constituents, 9-2-Methyl-Z, Z-3, 13 octadecadienol (terpenoid), 9,17-Octadecadienal, (Z)-, Nonanoic acid, 9-oxo-, methyl ester, 9,12-Octadecadienoic acid Z,Z and Octasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11,13,13,15,15-hexadecamethyl- have insecticidal exercise beside many different organic actions as recorded from quite a lot of botanical extracts. Whereas the constituents like Hexadecanoic acid, methyl ester and cis-9-Hexadecenal, each of them are larvicidal, cis-Vaccenic acid and 9-Oxononanoic acid exhibiting solely an insecticidal exercise beside Undecanoic acid the mosquito repellent. The opposite six constituents Linoelaidic acid, Oleic Acid, Z-2-Octadecen-1-ol, 1-Methoxy-3-hydroxymethylheptane, Cis-11,14-Eicosadieonic acid-methyl ester and Heptasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11,13,13-tetradecamethyl- are constituents of different plant extracts which confirmed as a complete an insecticidal exercise.
 anti-LIF |
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| YF-PA12952 | Abfrontier | 50 ug | EUR 435.6 |
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Description: Mouse polyclonal to LIF |
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 Rabbit Polyclonal antibody Anti-CRBN |
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| Anti-CRBN | ImmunoStep | 50 µg | EUR 418.8 |
 ELISA Kit) Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| DLR-LIF-p-48T | DL Develop | 48T | EUR 656.4 |
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Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| DLR-LIF-p-96T | DL Develop | 96T | EUR 858 |
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Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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 ELISA Kit) Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| DLR-LIF-Ra-48T | DL Develop | 48T | EUR 609.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| DLR-LIF-Ra-96T | DL Develop | 96T | EUR 793.2 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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 ELISA Kit) Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| DLR-LIF-b-48T | DL Develop | 48T | EUR 656.4 |
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Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| DLR-LIF-b-96T | DL Develop | 96T | EUR 858 |
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Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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 ELISA Kit) Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| DLR-LIF-c-48T | DL Develop | 48T | EUR 632.4 |
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Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| DLR-LIF-c-96T | DL Develop | 96T | EUR 825.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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 ELISA Kit) Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| DLR-LIF-Hu-48T | DL Develop | 48T | EUR 456 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| DLR-LIF-Hu-96T | DL Develop | 96T | EUR 582 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RDR-LIF-b-48Tests | Reddot Biotech | 48 Tests | EUR 696 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RDR-LIF-b-96Tests | Reddot Biotech | 96 Tests | EUR 968.4 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RDR-LIF-c-48Tests | Reddot Biotech | 48 Tests | EUR 668.4 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RDR-LIF-c-96Tests | Reddot Biotech | 96 Tests | EUR 928.8 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RDR-LIF-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 459.6 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RDR-LIF-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 630 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RDR-LIF-p-48Tests | Reddot Biotech | 48 Tests | EUR 696 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RDR-LIF-p-96Tests | Reddot Biotech | 96 Tests | EUR 968.4 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RDR-LIF-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 640.8 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RDR-LIF-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 890.4 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RD-LIF-b-48Tests | Reddot Biotech | 48 Tests | EUR 666 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RD-LIF-b-96Tests | Reddot Biotech | 96 Tests | EUR 925.2 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RD-LIF-c-48Tests | Reddot Biotech | 48 Tests | EUR 639.6 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RD-LIF-c-96Tests | Reddot Biotech | 96 Tests | EUR 888 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RD-LIF-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 440.4 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RD-LIF-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 602.4 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RD-LIF-p-48Tests | Reddot Biotech | 48 Tests | EUR 666 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RD-LIF-p-96Tests | Reddot Biotech | 96 Tests | EUR 925.2 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RD-LIF-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 613.2 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
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| RD-LIF-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 850.8 |
 LIF, mouse |
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| MO16102 | Neuromics | 100 ug | EUR 810 |
 LIF, Mouse |
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| HY-P7084 | MedChemExpress | 50ug | EUR 639.6 |
 Anti-LIF Antibody |
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| A01400 | BosterBio | 100ug/vial | EUR 352.8 |
 anti- LIF antibody |
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| LSMab09804 | Lifescience Market | 100 ug | EUR 463.2 |
anti- LIF antibody |
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| FNab09804 | FN Test | 100µg | EUR 658.5 |
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Description: Antibody raised against LIF |
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Anti-LIF Antibody |
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| PA1562 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-LIF Antibody |
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| PB9036 | BosterBio | 100ug/vial | EUR 400.8 |
 Anti-LIF antibody |
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| STJ96472 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to LIF. |
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Anti-LIF antibody |
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| STJ29846 | St John's Laboratory | 100 µl | EUR 332.4 |
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Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
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Anti-LIF antibody |
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| STJ73631 | St John's Laboratory | 100 µg | EUR 430.8 |
 antibody) Anti-LIF (aa28-39) antibody |
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| STJ72919 | St John's Laboratory | 100 µg | EUR 430.8 |
 Mouse LIF shRNA Plasmid |
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| 20-abx971321 | Abbexa |
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 Mouse LIF ELISA Kit |
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| EML0014 | Abclonal | 96Tests | EUR 625.2 |
 Recombinant Mouse LIF Protein |
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| R01400-1 | BosterBio | 100ug/vial | EUR 352.8 |
 Polyclonal Goat anti-GST α-form |
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| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 336 |
 Polyclonal Goat anti-GST μ-form |
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| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 336 |
 Polyclonal Goat anti-GST p-form |
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| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 336 |
 LIF Antibody |
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| 6245-002mg | ProSci | 0.02 mg | EUR 206.18 |
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Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation. |
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LIF Antibody |
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| 6245-01mg | ProSci | 0.1 mg | EUR 523.7 |
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Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation. |
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 LIF Peptide |
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| 6245P | ProSci | 0.05 mg | EUR 197.7 |
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Description: (IN) LIF peptide |
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 LIF antibody |
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| 70R-12299 | Fitzgerald | 100 ug | EUR 483.6 |
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Description: Rabbit polyclonal LIF antibody |
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LIF Peptide |
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| 43-200P | ProSci | 0.1 mg | EUR 405.6 |
LIF antibody |
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| 70R-14100 | Fitzgerald | 100 ug | EUR 386.4 |
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Description: Affinity purified Rabbit polyclonal LIF antibody |
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LIF antibody |
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| 70R-51295 | Fitzgerald | 100 ul | EUR 292.8 |
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Description: Purified Polyclonal LIF antibody |
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 LIF protein |
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| 30R-2771 | Fitzgerald | 10 ug | EUR 442.8 |
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Description: Purified recombinant Human LIF protein |
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LIF protein |
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| 30R-2772 | Fitzgerald | 10 ug | EUR 586.8 |
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Description: Purified recombinant Mouse LIF protein |
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LIF Antibody |
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| 36950-100ul | SAB | 100ul | EUR 302.4 |
LIF Antibody |
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| 25283-100ul | SAB | 100ul | EUR 468 |
 LIF siRNA |
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| 20-abx922546 | Abbexa |
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LIF siRNA |
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| 20-abx922547 | Abbexa |
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LIF antibody |
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| 70R-6230 | Fitzgerald | 50 ug | EUR 560.4 |
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Description: Rabbit polyclonal LIF antibody |
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 rHu LIF |
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| AK9977-0005 | Akron Biotech | 5µg | Ask for price |
rHu LIF |
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| AK9977-0025 | Akron Biotech | 25µg | Ask for price |
rHu LIF |
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| AK9977-0100 | Akron Biotech | 100µg | Ask for price |
