lcabie

Potential of chitosan/alginate nanoparticles as a non-viral vector for gene delivery: Formulation and optimization using D-optimal design

Chitosan/alginate (Chi/Alg) nanoparticles as a non-viral vector for the Smad4 encoding plasmid have been optimized using D-optimal design primarily based on the nanoparticles/plasmid ratio, Chi/Alg MW, and preparation technique sort. Following the optimization and validation of the most effective formulation, morphology research and FTIR measurements have been carried out to guage the optimized Chi/Alg/S NPs. Toxicity (MTT assay) and transfection research have been carried out for the most effective formulation as compared with Lipofectamine 2000, and Polyethyleneimine (PEI) and evaluated utilizing Inexperienced Fluorescence Protein (GFP) assay, Move cytometry, and RT-PCR.
The mannequin predicted a particle measurement of 111 nm, loading efficacy (LE) of 43%, cumulative launch (CMR) of 39%, the ζ-potential of +50 mV, and PDI of 0.13. The anticipated level situation was as follows: NP ratio = 13, Chi/Alg MW ratio = 2.35, and preparation technique sort = 1. Microscopic findings revealed that the form of nanoparticles was spherical. The Chi/Alg/S nanoparticles confirmed no toxicity and transfection efficacy of 29.9% was noticed as compared with Lipofectamine (35.5%) and PEI (30.9%).

Spatial distribution and habitat choice of culicoides imicola: The potential vector of bluetongue virus in Tunisia

The rising menace of vector-borne illnesses (VBDs) represents an awesome problem to those that handle public and animal well being. Figuring out the spatial distribution of arthropod vector species is an important step in learning the danger of transmission of a vector-borne pathogen (VBP) and in estimating threat ranges of VBD. Threat maps enable higher focusing on surveillance and assist in designing management measures.
We aimed to review the geographical distribution of Culicoides imicola, the primary competent vector of Bluetongue virus (BTV) in sheep in Tunisia. Fifty-three information overlaying the entire distribution vary of C.imicola in Tunisia have been obtained throughout a 2-year subject entomological survey (August 2017 – January 2018 and August 2018 – January 2019).
The ecological area of interest of C. imicola is described utilizing ecological-niche issue evaluation (ENFA) and Mahalanobis distances issue evaluation (MADIFA). An environmental suitability map (ESM) was developed by MaxEnt software program to map the optimum habitat below the present local weather background. The MaxEnt mannequin was extremely correct with a statistically vital space below curve (AUC) worth of 0.941. The situation of the potential distribution of C. imicola is predicted in specified areas of Tunisia. Our findings might be utilized in varied methods akin to surveillance and management program of BTV in Tunisia.
lcabie
lcabie

Analysis of a brand new viral vaccine vector in mice and rhesus macaques: J paramyxovirus (JPV)-expressing HA of influenza A virus H5N1

H5N1, an avian influenza virus, is thought to flow into in lots of Asian nations like Bangladesh, China, Cambodia, Indonesia, and Vietnam. The present FDA-approved H5N1 vaccine has a reasonable degree of efficacy. A protected and efficient vaccine is required to stop the outbreaks of extremely pathogenic avian influenza (HPAI) H5N1 in people. Non-segmented negative-sense single-stranded viruses (NNSVs) are broadly used as a vector to develop vaccines for people, animals, and poultry. NNSVs stably categorical international genes with out integrating with the host genome. J Paramyxovirus (JPV) is a non-segmented negative-strand RNA virus and a member of the proposed genus Jeilongvirus within the household Paramyxoviridae. JPV-specific antibodies have been detected in rodents, bats, people, and pigs, however the virus just isn’t related to illness in any species apart from mice. JPV replicates within the respiratory tract of mice and effectively expresses the virus-vectored international genes in tissue tradition cells. On this work, we explored JPV as a vector for creating an H5N1 vaccine utilizing intranasal supply. We integrated hemagglutinin (HA) of H5N1 into the JPV genome by changing the small hydrophobic (SH) gene to generate a recombinant JPV expressing HA (rJPV-ΔSH-H5). A single intranasal administration of rJPV-ΔSH-H5 protected mice from a deadly HPAI H5N1 problem. Intranasal vaccination of rJPV-ΔSH-H5 in rhesus macaques elicited antigen-specific humoral and cell-mediated immune responses. This work demonstrates that JPV is a promising vaccine vector. IMPORTANCE HPAI H5N1 outbreak in Southeast Asia destroyed hundreds of thousands of birds. Transmission of H5N1 into people resulted in deaths in lots of nations. On this work, we developed a novel H5N1 vaccine candidate utilizing JPV as a vector and demonstrated that JPV is an efficacious vaccine vector in animals. NNSVs stably categorical international genes with out integrating into the host genome. JPV, an NNSV, replicates effectively within the respiratory tract and induces strong immune responses.

Agro-waste derived compounds (flax and black seed peels): Toxicological impact towards the West Nile virus vectorCulex pipiens L. with particular reference to GC-MS evaluation

The event of various approaches to make use of agricultural residues as a supply of excessive value-added merchandise, develop into a should, particularly after the issues emerged as a consequence of their accumulation. This contribution demonstrates the potential of agricultural residues, Linuim usitatissium (flax seed) and Nigella sativa (black seed) peels, as uncooked supplies for the manufacturing of bioactive merchandise, botanical pesticides, towards Cx. pipiens, with deep evaluation to their chemical constituents by gasoline chromatography-mass spectrometry, the larvicidal efficacies of the three crude extracts (methylene chloride, petroleum ether and methanol 70%) from the 2 plant waste peels have been evaluated for the primary time towards the late third instar larvae of Cx. pipiens. Outcomes indicated completely different deadly doses in larvae relying on the efficacy of natural solvent used.
For each compounds methanol 70% extracts produced the best dry yield. Essentially the most environment friendly solvent is petroleum ether in case of each flax and Black seed peels. Petroleum ether extract exhibited the best toxicity towards Cx. pipiens with an LC50 of 69.6383 ppm. The identical outcomes for black seed indicated that petroleum ether was essentially the most environment friendly towards Cx. pipiens with an LC50 of 40.7748 ppm. The examine revealed for the primary time the kind of phytochemical constituents presents in peels of flax and black seeds utilizing GC-MS evaluation which revealed twenty-eight constituents amongst extracts of flax and black seed peels starting from to 58.8711% to 99.99% of the whole extracts. GC-MS profiling confirmed {that a} 5 constituents, 9-2-Methyl-Z, Z-3, 13 octadecadienol (terpenoid), 9,17-Octadecadienal, (Z)-, Nonanoic acid, 9-oxo-, methyl ester, 9,12-Octadecadienoic acid Z,Z and Octasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11,13,13,15,15-hexadecamethyl- have insecticidal exercise beside many different organic actions as recorded from quite a lot of botanical extracts. Whereas the constituents like Hexadecanoic acid, methyl ester and cis-9-Hexadecenal, each of them are larvicidal, cis-Vaccenic acid and 9-Oxononanoic acid exhibiting solely an insecticidal exercise beside Undecanoic acid the mosquito repellent. The opposite six constituents Linoelaidic acid, Oleic Acid, Z-2-Octadecen-1-ol, 1-Methoxy-3-hydroxymethylheptane, Cis-11,14-Eicosadieonic acid-methyl ester and Heptasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11,13,13-tetradecamethyl- are constituents of different plant extracts which confirmed as a complete an insecticidal exercise.

anti-LIF

YF-PA12952 50 ug
EUR 363
Description: Mouse polyclonal to LIF

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-b-48Tests 48 Tests
EUR 555

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-b-96Tests 96 Tests
EUR 771

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-c-48Tests 48 Tests
EUR 533

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-c-96Tests 96 Tests
EUR 740

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Hu-48Tests 48 Tests
EUR 367

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Hu-96Tests 96 Tests
EUR 502

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-p-48Tests 48 Tests
EUR 555

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-p-96Tests 96 Tests
EUR 771

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Ra-48Tests 48 Tests
EUR 511

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Ra-96Tests 96 Tests
EUR 709

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-b-48T 48T
EUR 547
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-b-96T 96T
EUR 715
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-c-48T 48T
EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-c-96T 96T
EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Hu-48T 48T
EUR 380
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Hu-96T 96T
EUR 485
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-p-48T 48T
EUR 547
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-p-96T 96T
EUR 715
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Ra-48T 48T
EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Ra-96T 96T
EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-b-48Tests 48 Tests
EUR 580

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-b-96Tests 96 Tests
EUR 807

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-c-48Tests 48 Tests
EUR 557

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-c-96Tests 96 Tests
EUR 774

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Hu-48Tests 48 Tests
EUR 383

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Hu-96Tests 96 Tests
EUR 525

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-p-48Tests 48 Tests
EUR 580

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-p-96Tests 96 Tests
EUR 807

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Ra-48Tests 48 Tests
EUR 534

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Ra-96Tests 96 Tests
EUR 742

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-LIF Antibody

PA1562 100ug/vial
EUR 294

Anti-LIF Antibody

PB9036 100ug/vial
EUR 334

Anti-LIF antibody

STJ29846 100 µl
EUR 277
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Anti-LIF Antibody

A01400 100ug/vial
EUR 294

Anti-LIF antibody

STJ96472 200 µl
EUR 197
Description: Rabbit polyclonal to LIF.

Anti-LIF antibody

STJ73631 100 µg
EUR 359

anti- LIF antibody

FNab09804 100µg
EUR 548.75
Description: Antibody raised against LIF

anti- LIF antibody

LSMab09804 100 ug
EUR 386

LIF, Mouse

HY-P7084 50ug
EUR 533

LIF, mouse

MO16102 100 ug
EUR 675

Anti-LIF (aa28-39) antibody

STJ72919 100 µg
EUR 359

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Recombinant Mouse LIF Protein

R01400-1 100ug/vial
EUR 294

Mouse LIF ELISA Kit

EML0014 96Tests
EUR 521

Mouse LIF shRNA Plasmid

20-abx971321
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

LIF Antibody

1-CSB-PA198481
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100

LIF Antibody

1-CSB-PA012928ESR2HU
  • EUR 222.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA

LIF Antibody

1-CSB-PA004761
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

LIF, Human

HY-P7049 10ug
EUR 1600

LIF, human

MO16101 100 ug
EUR 688

LIF Antibody

36950-100ul 100ul
EUR 252

LIF Antibody

25283-100ul 100ul
EUR 390

LIF siRNA

20-abx922546
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

LIF siRNA

20-abx922547
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

rHu LIF

AK9977-0005 5µg Ask for price

rHu LIF

AK9977-0025 25µg Ask for price

rHu LIF

AK9977-0100 100µg Ask for price

rHu LIF

AK9977-1000 1mg Ask for price

LIF antibody

PAab09804 100 ug
EUR 386

LIF Antibody

5216-100
EUR 316

LIF Antibody

5216-30T
EUR 146

LIF protein

30R-2771 10 ug
EUR 369
Description: Purified recombinant Human LIF protein