lcabie

Pre-retinal delivery of recombinant adeno-associated virus vector significantly improves retinal transduction efficiency

Intravitreal injection is essentially the most extensively used injection method for ocular gene supply. Nevertheless, vector diffusion is attenuated by bodily boundaries and neutralizing antibodies within the vitreous. The 13-lined floor squirrel (13-LGS), as in people, has a bigger relative vitreous physique quantity than the extra widespread rodent fashions corresponding to rats and mice, which might additional cut back transduction effectivity with the intravitreal injection route. We report right here a “pre-retinal” injection strategy that results in detachment of the posterior hyaloid membrane and delivers vector into the area between vitreous and interior retina.
Vectors carrying a ubiquitously expressing mCherry reporter have been injected into the deep vitreous or pre-retinal area in grownup wild-type 13-LGSs. Then, adeno-associated virus (AAV)-mediated mCherry expression was evaluated with non-invasive imaging, immunofluorescence, and stream cytometry. In comparison with deep vitreous supply, pre-retinal administration achieved pan-retinal gene expression with a decrease vector dose quantity and considerably elevated the variety of transduced cone photoreceptors.
These outcomes counsel that pre-retinal injection is a promising software within the growth of gene remedy methods in animal fashions and is a possible strategy to be used in human analysis, notably in youthful people with an intact posterior hyaloid membrane and secure vitreous.

A delicate and reproducible cell-based assay through secNanoLuc to detect neutralizing antibody towards adeno-associated virus vector capsid

Most gene remedy medical trials that systemically administered adeno-associated virus (AAV) vector enrolled solely sufferers with out anti-AAV-neutralizing antibodies. Nevertheless, laboratory exams to measure neutralizing antibodies various amongst medical trials and haven’t been standardized. On this research, we tried to enhance the sensitivity and reproducibility of a cell-based assay to detect neutralizing antibodies and to find out the detection threshold to foretell remedy efficacy.
Utility of the secreted sort of NanoLuc and AAV receptor-expressing cells diminished the multiplicity of an infection (MOI) for AAV transduction and improved the sensitivity to detect neutralizing antibodies with a low coefficient of variation, whereas the detection threshold couldn’t be improved by the discount of MOI to <100. After human immunoglobulin administration into mice at numerous doses, remedy with high-dose AAV8 vector enabled evasion of the inhibitory impact of neutralizing antibodies.
Conversely, gene transduction was barely influenced within the mice handled with low-dose AAV8 vector, even when neutralizing antibodies have been decided to be unfavorable within the assay. In conclusion, we developed a dependable and delicate cell-based assay to measure neutralizing antibodies towards AAV and located that the suitable MOI to detect marginal neutralizing antibodies was 100. Different components, together with noninhibitory antibodies, marginally affect in vivo transduction at low vector doses.
lcabie
lcabie

Bilateral Immune-Mediated Keratolysis After Immunization With SARS-CoV-2 Recombinant Viral Vector Vaccine

Goal: The aim of this research was to report an uncommon case of bilateral immune-mediated corneal melting and necrosis after ChAdOx1 nCoV-19 (Covishield) vaccination.
Strategies: This can be a case report and literature assessment.
Outcomes: A 48-year-old man introduced to the ophthalmic emergency division with progressive bilateral corneal melting 5 weeks after receiving the primary dose of ChAdOx1 nCoV-19 (Covishield) vaccine. Systemic complaints of fever, diarrhea, and vomiting have been famous within the first 2 weeks, which subsided earlier than the onset of ocular signs at day 21 of vaccine administration.
The affected person may solely understand mild bilaterally and demonstrated options of bilateral keratolysis with choroidal detachment on ultrasonography. The microbiological scraping specimen didn’t reveal development of any microorganism. Tectonic penetrating keratoplasty was carried out, and the host corneal tissue was despatched for histopathology, bacterial tradition, fungal tradition, polymerase chain response for herpes simplex virus, varicella zoster virus, cytomegalovirus, adenovirus, and SARS-CoV-2. Microbial tradition was sterile, and viral polymerase chain response stories have been unfavorable.
Histopathological examination revealed dense inflammatory cell infiltration. Detailed systemic workup revealed no underlying systemic or autoimmune pathology.
Conclusions: Immune-mediated keratolysis after ChAdOx1 nCoV-19 (Covishield) vaccination is a uncommon entity, and we consider that that is the primary report of a temporal affiliation between a severe ocular hostile occasion after a single dose of any SARS-CoV-19 vaccine. It might be included as a potential hostile occasion related to this vaccine.

Manufacturing of therapeutic iduronate-2-sulfatase enzyme with a novel single-stranded RNA virus vector

The Sendai virus vector has obtained plenty of consideration as a result of its broad tropism for mammalian cells. Because of efforts for genetic research primarily based on a mutant virus, we will now categorical greater than 10 genes of as much as 13.5 kilo nucleotides in a single vector with excessive protein expression effectivity. To show this profit, we examined the efficacy of the novel ribonucleic acid (RNA) virus vector harboring the human iduronate-2-sulfatase (IDS) gene with 1,653 base pairs, a causative gene for mucopolysaccharidosis sort II, also called a dysfunction of lysosomal storage issues.

As anticipated, this novel RNA vector with the human IDS gene exhibited its marked expression as decided by the expression of enhanced inexperienced fluorescent protein and IDS enzyme exercise. Whereas these cells exhibited a standard development fee, the BHK-21 transformant cells stably expressing the human IDS gene persistently generated an lively human IDS enzyme extracellularly. The human IDS protein produced didn’t be included into the lysosome when cells have been pretreated with mannose-6-phosphate, demonstrating that this human IDS enzyme has potential for therapeutic use by cross-correction. These outcomes counsel that our novel RNA vector could also be relevant for additional medical settings.

MVAΔ008 viral vector encoding the mannequin protein OVA induces improved immune response towards the heterologous antigen and equal ranges of safety in a mice tumor mannequin than the standard MVA

 

Modified vaccinia Ankara virus (MVA) is extensively used as a vaccine vector. Now we have beforehand noticed that MVAΔ008, an MVA missing the gene that codes for interleukin-18 binding protein, considerably will increase CD8+ and CD4+ T-cell responses to vaccinia virus (VACV) epitopes and recombinant HIV antigens. Nevertheless, the efficacy of this vector towards pathogens or tumor cells stays unclear.
Thus, the intention of this research was to judge the mobile immune response and the safety induced by recombinant MVAs encoding the mannequin antigen ovalbumin (OVA). We used the MO5 melanoma tumor mannequin (OVA-expressing tumor) as an strategy for evaluating the vector-induced efficacy. Our outcomes present that MVAΔ008-OVA (optimized vector) induced greater in vivo particular cytotoxicity and ex vivo T-cell IFN-γ responses towards OVA than the standard MVA vector.
Importantly, the recombinant vectors have been able to controlling MO5 tumor development. Certainly, the administration of MVAΔ008-OVA or MVA-OVA in prophylactic and therapeutic schemes offered whole safety and longer survival of mice, respectively. Total, our outcomes reveal the improved immunogenicity and the protecting capability of MVAΔ008 towards a heterologous mannequin antigen. These findings counsel that MVAΔ008 constitutes a superb candidate for vaccine growth towards pathogens or most cancers remedy.

anti-Galectin 1

YF-PA12945 100 ug
EUR 483.6
Description: Rabbit polyclonal to Galectin 1

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

Human Galectin-1 (LGALS1)

1-CSB-EP012882HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli

Human Galectin-1 (LGALS1)

1-CSB-EP012882HUb1
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli

anti- Galectin-1 antibody

FNab03314 100µg
EUR 606.3
Description: Antibody raised against Galectin-1

anti- Galectin-1 antibody

FNab03315 100µg
EUR 658.5
Description: Antibody raised against Galectin-1

Anti-Galectin 1 Antibody

A00470-2 100ug/vial
EUR 352.8

Anti-Galectin-1 antibody

PAab03314 100 ug
EUR 426

Anti-Galectin-1 antibody

STJ93201 200 µl
EUR 236.4
Description: Rabbit polyclonal to Galectin-1.

Anti-Galectin-1 antibody

STJ98716 200 µl
EUR 236.4
Description: Rabbit polyclonal to Galectin-1.

Anti-Galectin 1 (1A8)

YF-MA13984 100 ug
EUR 435.6
Description: Mouse monoclonal to Galectin 1

Anti-Galectin 1/Lgals1 Antibody

A00470 100ug/vial
EUR 400.8

Anti-Galectin 1 Biotinylated Antibody

A00470-Biotin 50ug/vial
EUR 352.8

Anti-Galectin 1/LGALS1 Antibody

PA1422 100ug/vial
EUR 400.8

anti-Galectin 1 (1E8-1B2)

LF-MA10174 100 ug
EUR 435.6
Description: Mouse monoclonal to Galectin 1

Anti-Galectin 1/LGALS1 Antibody

PB9240 100ug/vial
EUR 400.8

Anti-galectin-1 (mouse) antibody

STJ72519 100 µg
EUR 430.8

HRP-Goat Anti-Mouse Secondary Antibody

A12003
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

HRP-Goat Anti-Rabbit Secondary Antibody

A12004
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

Anti-Galectin-13 (GAL13) / Placental Protein 13 (PP13) Monoclonal Antibody

M08143-1 100ug/vial
EUR 476.4
Description: Mouse Monoclonal Galectin-13 (GAL13) / Placental Protein 13 (PP13) Antibody. Validated in IHC and tested in Human.

Goat Anti-Rabbit Secondary Antibody, Biotin Conjugated

A12001
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

Goat Anti-Rat Secondary Antibody, Biotin Conjugated

A12002
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

LGALS3 Human, Galectin-3 Human Recombinant Protein, His Tag

PROTP17931-1 Regular: 25ug
EUR 380.4
Description: LGALS3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 270 amino acids (1-250 a.a.) and having a molecular mass of 28.3 kDa._x000D_ The LGALS3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. _x000D_

LGALS7 Human, Galectin-7 Human Recombinant Protein, His Tag

PROTP47929-1 Regular: 20ug
EUR 380.4
Description: Galectin-7 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 156 amino acids (1-136 a.a.) and having a molecular mass of 17.2kDa. ;The Galectin-7 is purified by proprietary chromatographic techniques.

LGALS8 Human, Galectin-8 Human Recombinant Protein, His Tag

PROTO00214-1 Regular: 10ug
EUR 380.4
Description: LGALS8 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 337 amino acids (1-317 a.a.) and having a molecular mass of 37.9 kDa. The LGALS8 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Human Galectin-3 (Gal-3) AssayMax ELISA Kit

EG3311-1 96 Well Plate
EUR 572.4

Human Galectin-4 (Gal-4) AssayMax ELISA Kit

EG3312-1 96 Well Plate
EUR 572.4

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

anti-Galectin 13

YF-PA18585 50 ug
EUR 435.6
Description: Mouse polyclonal to Galectin 13

anti-Galectin 3

YF-PA24079 50 ul
EUR 400.8
Description: Mouse polyclonal to Galectin 3

anti-galectin 9

YF-PA24081 50 ul
EUR 400.8
Description: Mouse polyclonal to galectin 9

anti-Galectin 3

YF-PA12946 50 ug
EUR 435.6
Description: Mouse polyclonal to Galectin 3

anti-Galectin 3

YF-PA12947 100 ul
EUR 483.6
Description: Rabbit polyclonal to Galectin 3

anti-Galectin 3

YF-PA12948 100 ug
EUR 483.6
Description: Rabbit polyclonal to Galectin 3

anti-Galectin 8

YF-PA12951 50 ul
EUR 435.6
Description: Mouse polyclonal to Galectin 8

Human Galectin-1(LGALS1) ELISA kit

1-CSB-EL012882HU
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Galectin-1(LGALS1) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Recombinant Human Galectin-1

7-00427 10µg Ask for price

Recombinant Human Galectin-1

7-00428 50µg Ask for price

Recombinant Human Galectin-1

7-00429 1mg Ask for price

Galectin-1, human recombinant

4648-10
EUR 170.4

Galectin-1, human recombinant

4648-1000
EUR 1977.6

Galectin-1, human recombinant

4648-50
EUR 314.4

Human Galectin 1 Protein

20-abx066725
  • EUR 744.00
  • EUR 326.40
  • EUR 2230.80
  • EUR 878.40
  • EUR 543.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Bovine Galectin-1 (LGALS1)

1-CSB-EP012882BO
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bovine Galectin-1(LGALS1) expressed in E.coli

Anti-human Galectin-3 antibody

STJ15100163 250 µg
EUR 403.2
Description: This monoclonal antibody is for studies of antigen expression in cells and tissue sections using immunocytochemistry and immunoprecipitation

Human Genomic DNA 

X11000
  • Ask for price
  • EUR 235.40
  • 0.2 ml
  • 0.2 ml

Human Brain Genomic DNA  

X11001
  • Ask for price
  • EUR 77.00
  • 10 µg
  • 10 ul

Human Galectin-2 (LGALS2)

1-CSB-EP012886HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Galectin-2(LGALS2) expressed in E.coli

Human Galectin-9 (LGALS9)

1-CSB-EP012895HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Galectin-9(LGALS9) expressed in E.coli

Human Galectin-7 (LGALS7)

1-CSB-RP097274h
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Galectin-7(LGALS7) expressed in E.coli

Human Galectin-9 (LGALS9)

1-CSB-YP012895HU
  • EUR 516.00
  • EUR 280.80
  • EUR 1809.60
  • EUR 770.40
  • EUR 1210.80
  • EUR 349.20
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Galectin-9(LGALS9) expressed in Yeast

anti- Galectin 2 antibody

FNab03310 100µg
EUR 702
Description: Antibody raised against Galectin 2

anti- Galectin 4 antibody

FNab03311 100µg
EUR 606.3
Description: Antibody raised against Galectin 4

anti- Galectin 8 antibody

FNab03312 100µg
EUR 606.3
Description: Antibody raised against Galectin 8

anti- Galectin 9 antibody

FNab03313 100µg
EUR 606.3
Description: Antibody raised against Galectin 9

anti- Galectin-3 antibody

FNab03316 100µg
EUR 606.3
Description: Antibody raised against Galectin-3

anti- Galectin-3 antibody

FNab03317 100µg
EUR 658.5
Description: Antibody raised against Galectin-3

anti- Galectin-7 antibody

FNab03318 100µg
EUR 606.3
Description: Antibody raised against Galectin-7

Anti-Galectin-9 Antibody

A03415 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Galectin-9 Antibody (LGALS9) detection.tested for WB in Human, Mouse, Rat.

anti-Galectin-3 (6G2)

LF-MA20336 100 ug
EUR 424.8
Description: Mouse monoclonal to Galectin-3

Anti-Galectin 2 antibody

PAab03310 100 ug
EUR 494.4

Anti-Galectin 4 antibody

PAab03311 100 ug
EUR 426

Anti-Galectin 8 antibody

PAab03312 100 ug
EUR 426

Anti-Galectin 9 antibody

PAab03313 100 ug
EUR 426

Anti-Galectin-3 antibody

PAab03316 100 ug
EUR 426

Anti-Galectin-7 antibody

PAab03318 100 ug
EUR 426

Anti-Galectin-3 antibody

STJ16100369 1 mL
EUR 1256.4

Anti-Galectin-3 antibody

STJ16100782 100 µg
EUR 864

Anti-Galectin-3 antibody

STJ180106 0.1 ml
EUR 254.4

Anti-Galectin 3 antibody

STJ190057 200 µl
EUR 236.4
Description: Unconjugated Mouse monoclonal to Galectin 3 (AS1A24)

Anti-Galectin 3 antibody

STJ73098 100 µg
EUR 430.8

Anti-Galectin-3 antibody

STJ96945 200 µl
EUR 236.4
Description: Galectin-3 is a protein encoded by the LGALS3 gene which is approximately 26,1 kDa. Galectin-3 is localised to the cytoplasm and nucleus. It is involved in NF-kappaB signalling, advanced glycosylation end product receptor signalling and cell adhesion. It is a galactose-specific lectin which binds IgE and may mediate the stimulation by CSPG4 of endothelial cells migration along with the alpha-3, beta-1 integrin. It is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. Galectin-3 is expressed mainly in the colonic epithelium and is also abundantly expressed in activated macrophages. Mutations in the LGALS3 gene may result in follicular adenoma and thyroid cancer. STJ96945 was developed from clone 6G2 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous galectin-3 proteins.

Anti-Galectin-3 antibody

STJ97549 200 µl
EUR 236.4
Description: Mouse monoclonal to Galectin-3 (6B8).

Anti-Galectin-3 antibody

STJ97550 200 µl
EUR 236.4
Description: Mouse monoclonal to Galectin-3 (8D7).

Anti-Galectin-3 antibody

STJ97551 200 µl
EUR 236.4
Description: Mouse monoclonal to Galectin-3 (5D9).

Anti-Galectin-3 antibody

STJ97601 200 µl
EUR 236.4
Description: Mouse monoclonal to Galectin-3 (2F9).

Anti-Galectin-3 antibody

STJ97604 200 µl
EUR 236.4
Description: Mouse monoclonal to Galectin-3 (16E6).

Anti-Galectin-3 antibody

STJ97605 200 µl
EUR 236.4
Description: Mouse monoclonal to Galectin-3 (1H4).

Anti-Galectin-3 antibody

STJ97715 200 µl
EUR 236.4
Description: Mouse monoclonal to Galectin-3.

Anti-Galectin-2 antibody

STJ93202 200 µl
EUR 236.4
Description: Rabbit polyclonal to Galectin-2.

Anti-Galectin-4 antibody

STJ93203 200 µl
EUR 236.4
Description: Rabbit polyclonal to Galectin-4.

Anti-Galectin-7 antibody

STJ93204 200 µl
EUR 236.4
Description: Rabbit polyclonal to Galectin-7.

Anti-Galectin-8 antibody

STJ93205 200 µl
EUR 236.4
Description: Rabbit polyclonal to Galectin-8.

Anti-Galectin-9 antibody

STJ93206 200 µl
EUR 236.4
Description: Rabbit polyclonal to Galectin-9.

Anti-Galectin 8 (3E5)

YF-MA13986 100 ug
EUR 435.6
Description: Mouse monoclonal to Galectin 8

Galectin 1 Antibody

DF2383 200ul
EUR 420

Galectin 1 Antibody

DF8089 200ul
EUR 420

rHu Galectin-1

AK8281-0010 10µg Ask for price