Crispr Nuclease Crispr Overview Crispr Pam Sequence Crispr Plasmid Crispr Plasmids Crispr Reagents Crispr Service Crispr Sgrna Crispr Software Crispr Tutorial Crispr Vector Custom Gene Synthesis Goat Hamster Kangaroo Killifish Leech Listeria Orangutan Pig Pigeon Plant Rat Yeast
Pre-retinal delivery of recombinant adeno-associated virus vector significantly improves retinal transduction efficiency
Intravitreal injection is essentially the most extensively used injection method for ocular gene supply. Nevertheless, vector diffusion is attenuated by bodily boundaries and neutralizing antibodies within the vitreous. The 13-lined floor squirrel (13-LGS), as in people, has a bigger relative vitreous physique quantity than the extra widespread rodent fashions corresponding to rats and mice, which might additional cut back transduction effectivity with the intravitreal injection route. We report right here a “pre-retinal” injection strategy that results in detachment of the posterior hyaloid membrane and delivers vector into the area between vitreous and interior retina.
Vectors carrying a ubiquitously expressing mCherry reporter have been injected into the deep vitreous or pre-retinal area in grownup wild-type 13-LGSs. Then, adeno-associated virus (AAV)-mediated mCherry expression was evaluated with non-invasive imaging, immunofluorescence, and stream cytometry. In comparison with deep vitreous supply, pre-retinal administration achieved pan-retinal gene expression with a decrease vector dose quantity and considerably elevated the variety of transduced cone photoreceptors.
These outcomes counsel that pre-retinal injection is a promising software within the growth of gene remedy methods in animal fashions and is a possible strategy to be used in human analysis, notably in youthful people with an intact posterior hyaloid membrane and secure vitreous.
A delicate and reproducible cell-based assay through secNanoLuc to detect neutralizing antibody towards adeno-associated virus vector capsid
Most gene remedy medical trials that systemically administered adeno-associated virus (AAV) vector enrolled solely sufferers with out anti-AAV-neutralizing antibodies. Nevertheless, laboratory exams to measure neutralizing antibodies various amongst medical trials and haven’t been standardized. On this research, we tried to enhance the sensitivity and reproducibility of a cell-based assay to detect neutralizing antibodies and to find out the detection threshold to foretell remedy efficacy.
Utility of the secreted sort of NanoLuc and AAV receptor-expressing cells diminished the multiplicity of an infection (MOI) for AAV transduction and improved the sensitivity to detect neutralizing antibodies with a low coefficient of variation, whereas the detection threshold couldn’t be improved by the discount of MOI to <100. After human immunoglobulin administration into mice at numerous doses, remedy with high-dose AAV8 vector enabled evasion of the inhibitory impact of neutralizing antibodies.
Conversely, gene transduction was barely influenced within the mice handled with low-dose AAV8 vector, even when neutralizing antibodies have been decided to be unfavorable within the assay. In conclusion, we developed a dependable and delicate cell-based assay to measure neutralizing antibodies towards AAV and located that the suitable MOI to detect marginal neutralizing antibodies was 100. Different components, together with noninhibitory antibodies, marginally affect in vivo transduction at low vector doses.
Bilateral Immune-Mediated Keratolysis After Immunization With SARS-CoV-2 Recombinant Viral Vector Vaccine
Goal: The aim of this research was to report an uncommon case of bilateral immune-mediated corneal melting and necrosis after ChAdOx1 nCoV-19 (Covishield) vaccination.
Strategies: This can be a case report and literature assessment.
Outcomes: A 48-year-old man introduced to the ophthalmic emergency division with progressive bilateral corneal melting 5 weeks after receiving the primary dose of ChAdOx1 nCoV-19 (Covishield) vaccine. Systemic complaints of fever, diarrhea, and vomiting have been famous within the first 2 weeks, which subsided earlier than the onset of ocular signs at day 21 of vaccine administration.
The affected person may solely understand mild bilaterally and demonstrated options of bilateral keratolysis with choroidal detachment on ultrasonography. The microbiological scraping specimen didn’t reveal development of any microorganism. Tectonic penetrating keratoplasty was carried out, and the host corneal tissue was despatched for histopathology, bacterial tradition, fungal tradition, polymerase chain response for herpes simplex virus, varicella zoster virus, cytomegalovirus, adenovirus, and SARS-CoV-2. Microbial tradition was sterile, and viral polymerase chain response stories have been unfavorable.
Histopathological examination revealed dense inflammatory cell infiltration. Detailed systemic workup revealed no underlying systemic or autoimmune pathology.
Conclusions: Immune-mediated keratolysis after ChAdOx1 nCoV-19 (Covishield) vaccination is a uncommon entity, and we consider that that is the primary report of a temporal affiliation between a severe ocular hostile occasion after a single dose of any SARS-CoV-19 vaccine. It might be included as a potential hostile occasion related to this vaccine.
Manufacturing of therapeutic iduronate-2-sulfatase enzyme with a novel single-stranded RNA virus vector
The Sendai virus vector has obtained plenty of consideration as a result of its broad tropism for mammalian cells. Because of efforts for genetic research primarily based on a mutant virus, we will now categorical greater than 10 genes of as much as 13.5 kilo nucleotides in a single vector with excessive protein expression effectivity. To show this profit, we examined the efficacy of the novel ribonucleic acid (RNA) virus vector harboring the human iduronate-2-sulfatase (IDS) gene with 1,653 base pairs, a causative gene for mucopolysaccharidosis sort II, also called a dysfunction of lysosomal storage issues.
As anticipated, this novel RNA vector with the human IDS gene exhibited its marked expression as decided by the expression of enhanced inexperienced fluorescent protein and IDS enzyme exercise. Whereas these cells exhibited a standard development fee, the BHK-21 transformant cells stably expressing the human IDS gene persistently generated an lively human IDS enzyme extracellularly. The human IDS protein produced didn’t be included into the lysosome when cells have been pretreated with mannose-6-phosphate, demonstrating that this human IDS enzyme has potential for therapeutic use by cross-correction. These outcomes counsel that our novel RNA vector could also be relevant for additional medical settings.
MVAΔ008 viral vector encoding the mannequin protein OVA induces improved immune response towards the heterologous antigen and equal ranges of safety in a mice tumor mannequin than the standard MVA
Modified vaccinia Ankara virus (MVA) is extensively used as a vaccine vector. Now we have beforehand noticed that MVAΔ008, an MVA missing the gene that codes for interleukin-18 binding protein, considerably will increase CD8+ and CD4+ T-cell responses to vaccinia virus (VACV) epitopes and recombinant HIV antigens. Nevertheless, the efficacy of this vector towards pathogens or tumor cells stays unclear.
Thus, the intention of this research was to judge the mobile immune response and the safety induced by recombinant MVAs encoding the mannequin antigen ovalbumin (OVA). We used the MO5 melanoma tumor mannequin (OVA-expressing tumor) as an strategy for evaluating the vector-induced efficacy. Our outcomes present that MVAΔ008-OVA (optimized vector) induced greater in vivo particular cytotoxicity and ex vivo T-cell IFN-γ responses towards OVA than the standard MVA vector.
Importantly, the recombinant vectors have been able to controlling MO5 tumor development. Certainly, the administration of MVAΔ008-OVA or MVA-OVA in prophylactic and therapeutic schemes offered whole safety and longer survival of mice, respectively. Total, our outcomes reveal the improved immunogenicity and the protecting capability of MVAΔ008 towards a heterologous mannequin antigen. These findings counsel that MVAΔ008 constitutes a superb candidate for vaccine growth towards pathogens or most cancers remedy.
 anti-Galectin 1 |
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| YF-PA12945 | Abfrontier | 100 ug | EUR 483.6 |
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Description: Rabbit polyclonal to Galectin 1 |
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 Rabbit Polyclonal antibody Anti-CRBN |
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| Anti-CRBN | ImmunoStep | 50 µg | EUR 418.8 |
) Human Galectin-1 (LGALS1) |
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| 1-CSB-EP012882HU | Cusabio |
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Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli |
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Human Galectin-1 (LGALS1) |
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| 1-CSB-EP012882HUb1 | Cusabio |
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Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli |
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 anti- Galectin-1 antibody |
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| FNab03314 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against Galectin-1 |
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anti- Galectin-1 antibody |
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| FNab03315 | FN Test | 100µg | EUR 658.5 |
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Description: Antibody raised against Galectin-1 |
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 Anti-Galectin 1 Antibody |
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| A00470-2 | BosterBio | 100ug/vial | EUR 352.8 |
 Anti-Galectin-1 antibody |
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| PAab03314 | Lifescience Market | 100 ug | EUR 426 |
Anti-Galectin-1 antibody |
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| STJ93201 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to Galectin-1. |
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Anti-Galectin-1 antibody |
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| STJ98716 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to Galectin-1. |
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) Anti-Galectin 1 (1A8) |
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| YF-MA13984 | Abfrontier | 100 ug | EUR 435.6 |
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Description: Mouse monoclonal to Galectin 1 |
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 Anti-Galectin 1/Lgals1 Antibody |
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| A00470 | BosterBio | 100ug/vial | EUR 400.8 |
 Anti-Galectin 1 Biotinylated Antibody |
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| A00470-Biotin | BosterBio | 50ug/vial | EUR 352.8 |
 Anti-Galectin 1/LGALS1 Antibody |
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| PA1422 | BosterBio | 100ug/vial | EUR 400.8 |
) anti-Galectin 1 (1E8-1B2) |
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| LF-MA10174 | Abfrontier | 100 ug | EUR 435.6 |
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Description: Mouse monoclonal to Galectin 1 |
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Anti-Galectin 1/LGALS1 Antibody |
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| PB9240 | BosterBio | 100ug/vial | EUR 400.8 |
 antibody) Anti-galectin-1 (mouse) antibody |
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| STJ72519 | St John's Laboratory | 100 µg | EUR 430.8 |
 HRP-Goat Anti-Mouse Secondary Antibody |
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| A12003 | EpiGentek |
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 HRP-Goat Anti-Rabbit Secondary Antibody |
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| A12004 | EpiGentek |
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 / Placental Protein 13 (PP13) Monoclonal Antibody) Anti-Galectin-13 (GAL13) / Placental Protein 13 (PP13) Monoclonal Antibody |
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| M08143-1 | BosterBio | 100ug/vial | EUR 476.4 |
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Description: Mouse Monoclonal Galectin-13 (GAL13) / Placental Protein 13 (PP13) Antibody. Validated in IHC and tested in Human. |
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 Goat Anti-Rabbit Secondary Antibody, Biotin Conjugated |
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| A12001 | EpiGentek |
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 Goat Anti-Rat Secondary Antibody, Biotin Conjugated |
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| A12002 | EpiGentek |
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 LGALS3 Human, Galectin-3 Human Recombinant Protein, His Tag |
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| PROTP17931-1 | BosterBio | Regular: 25ug | EUR 380.4 |
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Description: LGALS3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 270 amino acids (1-250 a.a.) and having a molecular mass of 28.3 kDa._x000D_ The LGALS3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. _x000D_ |
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 LGALS7 Human, Galectin-7 Human Recombinant Protein, His Tag |
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| PROTP47929-1 | BosterBio | Regular: 20ug | EUR 380.4 |
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Description: Galectin-7 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 156 amino acids (1-136 a.a.) and having a molecular mass of 17.2kDa. ;The Galectin-7 is purified by proprietary chromatographic techniques. |
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 LGALS8 Human, Galectin-8 Human Recombinant Protein, His Tag |
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| PROTO00214-1 | BosterBio | Regular: 10ug | EUR 380.4 |
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Description: LGALS8 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 337 amino acids (1-317 a.a.) and having a molecular mass of 37.9 kDa. The LGALS8 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. |
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 Polyclonal Goat anti-GST μ-form |
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| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 336 |
 Polyclonal Goat anti-GST p-form |
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| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 336 |
 AssayMax ELISA Kit) Human Galectin-3 (Gal-3) AssayMax ELISA Kit |
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| EG3311-1 | AssayPro | 96 Well Plate | EUR 572.4 |
 AssayMax ELISA Kit) Human Galectin-4 (Gal-4) AssayMax ELISA Kit |
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| EG3312-1 | AssayPro | 96 Well Plate | EUR 572.4 |
 anti-Galectin 13 |
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| YF-PA18585 | Abfrontier | 50 ug | EUR 435.6 |
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Description: Mouse polyclonal to Galectin 13 |
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 anti-Galectin 3 |
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| YF-PA24079 | Abfrontier | 50 ul | EUR 400.8 |
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Description: Mouse polyclonal to Galectin 3 |
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 anti-galectin 9 |
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| YF-PA24081 | Abfrontier | 50 ul | EUR 400.8 |
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Description: Mouse polyclonal to galectin 9 |
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anti-Galectin 3 |
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| YF-PA12946 | Abfrontier | 50 ug | EUR 435.6 |
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Description: Mouse polyclonal to Galectin 3 |
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anti-Galectin 3 |
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| YF-PA12947 | Abfrontier | 100 ul | EUR 483.6 |
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Description: Rabbit polyclonal to Galectin 3 |
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anti-Galectin 3 |
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| YF-PA12948 | Abfrontier | 100 ug | EUR 483.6 |
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Description: Rabbit polyclonal to Galectin 3 |
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 anti-Galectin 8 |
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| YF-PA12951 | Abfrontier | 50 ul | EUR 435.6 |
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Description: Mouse polyclonal to Galectin 8 |
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 ELISA kit) Human Galectin-1(LGALS1) ELISA kit |
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| 1-CSB-EL012882HU | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Human Galectin-1(LGALS1) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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 Recombinant Human Galectin-1 |
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| 7-00427 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Galectin-1 |
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| 7-00428 | CHI Scientific | 50µg | Ask for price |
Recombinant Human Galectin-1 |
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| 7-00429 | CHI Scientific | 1mg | Ask for price |
 Human Galectin 1 Protein |
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| 20-abx066725 | Abbexa |
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 Galectin-1, human recombinant |
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| 4648-10 | Biovision | each | EUR 170.4 |
Galectin-1, human recombinant |
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| 4648-1000 | Biovision | each | EUR 1977.6 |
Galectin-1, human recombinant |
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| 4648-50 | Biovision | each | EUR 314.4 |
) Bovine Galectin-1 (LGALS1) |
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| 1-CSB-EP012882BO | Cusabio |
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Description: Recombinant Bovine Galectin-1(LGALS1) expressed in E.coli |
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 Anti-human Galectin-3 antibody |
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| STJ15100163 | St John's Laboratory | 250 µg | EUR 403.2 |
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Description: This monoclonal antibody is for studies of antigen expression in cells and tissue sections using immunocytochemistry and immunoprecipitation |
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 Human Genomic DNA |
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| X11000 | EpiGentek |
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 Human Brain Genomic DNA |
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| X11001 | EpiGentek |
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) Human Galectin-2 (LGALS2) |
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| 1-CSB-EP012886HU | Cusabio |
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Description: Recombinant Human Galectin-2(LGALS2) expressed in E.coli |
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) Human Galectin-9 (LGALS9) |
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| 1-CSB-EP012895HU | Cusabio |
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Description: Recombinant Human Galectin-9(LGALS9) expressed in E.coli |
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) Human Galectin-7 (LGALS7) |
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| 1-CSB-RP097274h | Cusabio |
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Description: Recombinant Human Galectin-7(LGALS7) expressed in E.coli |
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Human Galectin-9 (LGALS9) |
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| 1-CSB-YP012895HU | Cusabio |
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Description: Recombinant Human Galectin-9(LGALS9) expressed in Yeast |
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 anti- Galectin 2 antibody |
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| FNab03310 | FN Test | 100µg | EUR 702 |
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Description: Antibody raised against Galectin 2 |
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 anti- Galectin 4 antibody |
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| FNab03311 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against Galectin 4 |
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 anti- Galectin 8 antibody |
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| FNab03312 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against Galectin 8 |
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 anti- Galectin 9 antibody |
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| FNab03313 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against Galectin 9 |
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 anti- Galectin-3 antibody |
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| FNab03316 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against Galectin-3 |
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anti- Galectin-3 antibody |
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| FNab03317 | FN Test | 100µg | EUR 658.5 |
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Description: Antibody raised against Galectin-3 |
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 anti- Galectin-7 antibody |
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| FNab03318 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against Galectin-7 |
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 Anti-Galectin-9 Antibody |
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| A03415 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for Galectin-9 Antibody (LGALS9) detection.tested for WB in Human, Mouse, Rat. |
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) anti-Galectin-3 (6G2) |
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| LF-MA20336 | Abfrontier | 100 ug | EUR 424.8 |
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Description: Mouse monoclonal to Galectin-3 |
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 Anti-Galectin 2 antibody |
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| PAab03310 | Lifescience Market | 100 ug | EUR 494.4 |
 Anti-Galectin 4 antibody |
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| PAab03311 | Lifescience Market | 100 ug | EUR 426 |
 Anti-Galectin 8 antibody |
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| PAab03312 | Lifescience Market | 100 ug | EUR 426 |
 Anti-Galectin 9 antibody |
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| PAab03313 | Lifescience Market | 100 ug | EUR 426 |
 Anti-Galectin-3 antibody |
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| PAab03316 | Lifescience Market | 100 ug | EUR 426 |
 Anti-Galectin-7 antibody |
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| PAab03318 | Lifescience Market | 100 ug | EUR 426 |
Anti-Galectin-3 antibody |
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| STJ16100369 | St John's Laboratory | 1 mL | EUR 1256.4 |
Anti-Galectin-3 antibody |
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| STJ16100782 | St John's Laboratory | 100 µg | EUR 864 |
Anti-Galectin-3 antibody |
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| STJ180106 | St John's Laboratory | 0.1 ml | EUR 254.4 |
 Anti-Galectin 3 antibody |
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| STJ190057 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Unconjugated Mouse monoclonal to Galectin 3 (AS1A24) |
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Anti-Galectin 3 antibody |
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| STJ73098 | St John's Laboratory | 100 µg | EUR 430.8 |
Anti-Galectin-3 antibody |
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| STJ96945 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Galectin-3 is a protein encoded by the LGALS3 gene which is approximately 26,1 kDa. Galectin-3 is localised to the cytoplasm and nucleus. It is involved in NF-kappaB signalling, advanced glycosylation end product receptor signalling and cell adhesion. It is a galactose-specific lectin which binds IgE and may mediate the stimulation by CSPG4 of endothelial cells migration along with the alpha-3, beta-1 integrin. It is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. Galectin-3 is expressed mainly in the colonic epithelium and is also abundantly expressed in activated macrophages. Mutations in the LGALS3 gene may result in follicular adenoma and thyroid cancer. STJ96945 was developed from clone 6G2 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous galectin-3 proteins. |
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Anti-Galectin-3 antibody |
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| STJ97549 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Mouse monoclonal to Galectin-3 (6B8). |
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Anti-Galectin-3 antibody |
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| STJ97550 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Mouse monoclonal to Galectin-3 (8D7). |
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Anti-Galectin-3 antibody |
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| STJ97551 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Mouse monoclonal to Galectin-3 (5D9). |
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Anti-Galectin-3 antibody |
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| STJ97601 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Mouse monoclonal to Galectin-3 (2F9). |
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Anti-Galectin-3 antibody |
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| STJ97604 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Mouse monoclonal to Galectin-3 (16E6). |
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Anti-Galectin-3 antibody |
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| STJ97605 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Mouse monoclonal to Galectin-3 (1H4). |
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Anti-Galectin-3 antibody |
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| STJ97715 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Mouse monoclonal to Galectin-3. |
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 Anti-Galectin-2 antibody |
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| STJ93202 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to Galectin-2. |
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 Anti-Galectin-4 antibody |
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| STJ93203 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to Galectin-4. |
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Anti-Galectin-7 antibody |
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| STJ93204 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to Galectin-7. |
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 Anti-Galectin-8 antibody |
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| STJ93205 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to Galectin-8. |
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 Anti-Galectin-9 antibody |
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| STJ93206 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to Galectin-9. |
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) Anti-Galectin 8 (3E5) |
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| YF-MA13986 | Abfrontier | 100 ug | EUR 435.6 |
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Description: Mouse monoclonal to Galectin 8 |
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 Galectin 1 Antibody |
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| DF2383 | Affbiotech | 200ul | EUR 420 |
Galectin 1 Antibody |
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| DF8089 | Affbiotech | 200ul | EUR 420 |
 rHu Galectin-1 |
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| AK8281-0010 | Akron Biotech | 10µg | Ask for price |
