Tag: gene hackman
Development of dual-inducible duet-expression vectors for tunable gene expression control and CRISPR interference-based gene repression in Pseudomonas putida KT2440
The event of P. putida as an industrial host requires a complicated molecular toolbox for pressure enchancment, together with vectors for gene expression and repression. To enhance present expression plasmids for metabolic engineering, we developed a sequence of dual-inducible duet-expression vectors for P. putida KT2440. Various inducible promoters (Plac , Ptac , PtetR/tetA and Pdangerous ) had been utilized…
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Improved functionality and potency of next generation BinMLV viral vectors toward safer gene therapy
To develop safer retroviral murine leukemia virus (MLV)-based vectors, we beforehand mutated and re-engineered the MLV integrase: the W390A mutation abolished the interplay with its mobile tethering components, BET proteins, and a retargeting peptide (the chromodomain of the CBX1 protein) was fused C-terminally. The ensuing BET-independent MLVW390A-CBX was proven to combine effectively and extra randomly, away…
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Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative host Nicotiana excelsiana
Plant virus-based expression methods are another expression platform for the manufacturing of clinically and industrially helpful recombinant proteins. Nonetheless, because of a scarcity of viral vector with the industrial potentials, it’s pressing to design and develop new, versatile, and environment friendly plant virus vectors. The genome of Tomato bushy stunt virus (TBSV) provides…
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