Development of dual-inducible duet-expression vectors for tunable gene expression control and CRISPR interference-based gene repression in Pseudomonas putida KT2440

The event of P. putida as an industrial host requires a complicated molecular toolbox for pressure enchancment, together with vectors for gene expression and repression. To enhance present expression plasmids for metabolic engineering, we developed a sequence of dual-inducible duet-expression vectors for P. putida KT2440. Various inducible promoters (Plac , Ptac , PtetR/tetA and Pdangerous ) had been utilized…

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