Tag: generator
Development of dual-inducible duet-expression vectors for tunable gene expression control and CRISPR interference-based gene repression in Pseudomonas putida KT2440
The event of P. putida as an industrial host requires a complicated molecular toolbox for pressure enchancment, together with vectors for gene expression and repression. To enhance present expression plasmids for metabolic engineering, we developed a sequence of dual-inducible duet-expression vectors for P. putida KT2440. Various inducible promoters (Plac , Ptac , PtetR/tetA and Pdangerous ) had been utilized…
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Improved functionality and potency of next generation BinMLV viral vectors toward safer gene therapy
To develop safer retroviral murine leukemia virus (MLV)-based vectors, we beforehand mutated and re-engineered the MLV integrase: the W390A mutation abolished the interplay with its mobile tethering components, BET proteins, and a retargeting peptide (the chromodomain of the CBX1 protein) was fused C-terminally. The ensuing BET-independent MLVW390A-CBX was proven to combine effectively and extra randomly, away…
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