Improved functionality and potency of next generation BinMLV viral vectors toward safer gene therapy

To develop safer retroviral murine leukemia virus (MLV)-based vectors, we beforehand mutated and re-engineered the MLV integrase: the W390A mutation abolished the interplay with its mobile tethering components, BET proteins, and a retargeting peptide (the chromodomain of the CBX1 protein) was fused C-terminally. The ensuing BET-independent MLVW390A-CBX was proven to combine effectively and extra randomly, away…

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