A panel of real-time PCR assays for the detection of Bourbon virus, Heartland virus, West Nile virus, and Trypanosoma cruzi in major disease-transmitting vectors

• Samuel Wilson

Vector-borne pathogens, akin to Bourbon virus (BRBV), Heartland virus (HRTV), West Nile virus (WNV), and Trypanosoma cruzi (TCZ) are an excellent menace to public well being and animal well being. We developed a panel of TaqMan real-time PCR assays for pathogen surveillance. PCR targets had been chosen based mostly on nucleic acid sequences deposited in GenBank. Primers…