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Potential of chitosan/alginate nanoparticles as a non-viral vector for gene delivery: Formulation and optimization using D-optimal design
Chitosan/alginate (Chi/Alg) nanoparticles as a non-viral vector for the Smad4 encoding plasmid have been optimized using D-optimal design primarily based on the nanoparticles/plasmid ratio, Chi/Alg MW, and preparation technique sort. Following the optimization and validation of the most effective formulation, morphology research and FTIR measurements have been carried out to guage the optimized Chi/Alg/S NPs.…
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Pre-retinal delivery of recombinant adeno-associated virus vector significantly improves retinal transduction efficiency
Intravitreal injection is essentially the most extensively used injection method for ocular gene supply. Nevertheless, vector diffusion is attenuated by bodily boundaries and neutralizing antibodies within the vitreous. The 13-lined floor squirrel (13-LGS), as in people, has a bigger relative vitreous physique quantity than the extra widespread rodent fashions corresponding to rats and mice, which…
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