Crispr Nuclease Crispr Overview Crispr Pam Sequence Crispr Plasmid Crispr Plasmids Crispr Products Crispr Reagents Crispr Service Crispr Sgrna Crispr Transfection Crispr Vector Custom Gene Synthesis elisa Goat Insect Kangaroo Killifish Leech Listeria Orangutan Pig Pigeon Plant Rat
The impact of insecticides and plant extracts on the suppression of insect vector (Bemisia tabaci) of Mungbean yellow mosaic virus (MYMV)
Mungbean yellow mosaic virus (MYMV) is a crucial constraint in profitable manufacturing of mungbean (Vigna radiata L.) in lots of nations, together with Pakistan. The MYMV spreads by insect vector whitefly (Bemisia tabaci Gennadius). Using resistant cultivars is the simplest administration ways for MYMV. Twenty mungbean varieties/strains have been screened towards insect vector of MYMV below discipline situation within the present examine. Resistance ranges for varieties/strains have been assessed by visible scoring of typical illness signs.
Moreover, the impacts of two pesticides ‘Imidacloprid’ and ‘Thiamethoxam’ and two plant extracts, i.e., neem (Azadirachta indica), and Eucalyptus (Eucalyptus camaldulensis) have been examined on the suppression of whitefly. Subject screening indicated that not one of the examined varieties/strains proved immune/extremely resistant, whereas vital variations have been recorded amongst varieties/strains for resistance level. All varieties/strains have been systemically contaminated with MYMV. The varieties ‘AARI-2006’ and ‘Mung-14043’ have been thought of as proof against MYMV primarily based on visible signs and the bottom vector inhabitants. These varieties have been adopted by ‘NM-2006’ and ‘NL-31’, which proved as reasonably proof against MYMV.
All remaining varieties/strains have been grouped as reasonably to extremely prone to MYMV primarily based on visible signs’ scoring. These outcomes revealed that current mungbean germplasm don’t possess excessive resistance degree MYMV. Nonetheless, the strains displaying larger resistance within the present examine should be exploited in breeding applications for the event of resistant mungbean varieties/strains towards MYMV. Imidacloprid proved as the simplest insecticide in any respect concentrations to handle whitefly inhabitants. Subsequently, use of the varieties with larger resistance degree and spraying Imidacloprid may decrease the incidence of MYMV.
Transduction Effectivity and Immunogenicity of Viral Vectors for Cochlear Gene Remedy: A Systematic Assessment of Preclinical Animal Research
Background: Listening to impairment is probably the most frequent sensory deficit, affecting 466 million individuals worldwide and has been listed by the World Well being Group (WHO) as one of many precedence illnesses for analysis into therapeutic interventions to deal with public well being wants. Interior ear gene remedy is a promising method to revive sensorineural listening to loss, for which a number of gene remedy purposes have been studied and reported in preclinical animal research.
Systematic Assessment
To carry out a scientific evaluate on preclinical research reporting cochlear gene remedy, with a particular concentrate on transduction effectivity.
Strategies: An preliminary PubMed search was carried out on April 1st 2021 utilizing the PRISMA methodology. Preclinical in vivo research reporting main knowledge relating to transduction effectivity of gene remedy focusing on the internal ear have been included on this report.
Outcomes: Thirty-six research have been included on this evaluate. Transduction of varied cell sorts within the internal ear could be achieved, in keeping with the viral vector used. Nonetheless, there may be vital variability within the utilized vector supply techniques, together with promoter, viral vector titer, and so on.
Conclusion: Though gene remedy presents a promising method to deal with sensorineural listening to loss in preclinical research, the heterogeneity of methodologies impedes the identification of probably the most promising instruments for future use in internal ear therapies.
Towards Growth of Neuron Particular Transduction After Systemic Supply of Viral Vectors
Widespread transduction of the CNS with a single, non-invasive systemic injection of adeno-associated virus is now attainable because of the creation of blood-brain barrier-permeable capsids. Nonetheless, as these capsids are mutants of AAV9, they don’t have particular neuronal tropism. Subsequently, it’s essential to make use of genetic instruments to limit expression of the transgene to neuronal tissues. Right here we examine the power and specificity of two neuron-specific promoters, human synapsin 1 and mouse calmodulin/calcium dependent kinase II, to the ever present CAG promoter. Administration of a excessive titer of virus is important for widespread CNS transduction. We noticed the neuron-specific promoters drive comparable total expression within the mind to the CAG promoter. Moreover, the neuron-specific promoters confer considerably much less transgene expression in peripheral tissues in contrast with the CAG promoter. Future experiments will make the most of these supply platforms to over-express the Alzheimer-associated pathological proteins amyloid-beta and tau to create mouse fashions with out transgenesis.
Easy derivation of skeletal muscle from human pluripotent stem cells utilizing temperature-sensitive Sendai virus vector
Human pluripotent stem cells have the potential to distinguish into numerous cell sorts together with skeletal muscle tissues (SkM), and they’re utilized to regenerative drugs or in vitro modelling for intractable illnesses. A easy differentiation methodology is required for SkM cells to speed up neuromuscular illness research.
Right here, we established a easy methodology to transform human pluripotent stem cells into SkM cells through the use of temperature-sensitive Sendai virus (SeV) vector encoding myoblast dedication protein 1 (SeV-Myod1), a myogenic grasp transcription issue. SeV-Myod1 therapy transformed human embryonic stem cells (ESCs) into SkM cells, which expressed SkM markers together with myosin heavy chain (MHC).
We then eliminated the SeV vector by temporal therapy at a excessive temperature of 38℃, which additionally accelerated mesodermal differentiation, and located that SkM cells exhibited fibre-like morphology. Lastly, after elimination of the residual human ESCs by pluripotent stem cell-targeting supply of cytotoxic compound, we generated SkM cells with 80% MHC positivity and responsiveness to electrical stimulation. This easy methodology for myogenic differentiation was relevant to human-induced pluripotent stem cells and will probably be helpful for investigations of illness mechanisms and drug discovery sooner or later.
Broad and potent bispecific neutralizing antibody gene supply utilizing adeno-associated viral vectors for passive immunization towards HIV-1
Broadly neutralizing antibodies (bNAbs) possess favorable security, and passive immunization utilizing these can forestall or management human immunodeficiency virus sort 1 (HIV-1) an infection. Nonetheless, bNAbs typically used for monotherapy (IC80 > 5 μg/mL) have restricted breadth and efficiency and neutralize solely 70-90% of all HIV-1 strains. To handle the necessity for broader protection of the HIV-1 epidemic and improve the power of bNAbs to focus on HIV-1, we fused the single-chain variable antibody fragment (scFv) of bNAbs (PG9, PGT123, or NIH45-46) with full-length ibalizumab (iMab) in an scFv-monoclonal antibody tandem format to assemble bispecific bNAbs (BibNAbs).
Moreover, we described the feasibility of BibNAb gene supply mediated by recombinant adeno-associated virus 8 (rAAV8) for producing long-term expression with a single injection versus short-term passive immunization requiring steady injections. Our outcomes confirmed that the expressed BibNAbs focusing on two distinct epitopes exhibited neutralizing exercise towards 20 HIV-1 pseudoviruses in vitro.
After injecting a single rAAV8 vector, the expression and neutralizing exercise of the BibNAbs in serum have been sustained for 24 weeks. To the most effective of our information, only a few research have been revealed on BibNAb gene supply utilizing rAAV8 vectors towards HIV-1. BibNAb gene supply utilizing rAAV8 vectors could also be promising for passive immunization towards HIV-1 an infection.
 Anti-IL-8 antibody |
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| STJ98175 | St John's Laboratory | 100 µl | EUR 280.8 |
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Description: Mouse monoclonal to IL-8. |
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Anti-IL-8 antibody |
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| STJ97710 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Mouse monoclonal to IL-8. |
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Anti-IL-8 antibody |
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| STJ97717 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Mouse monoclonal to IL-8. |
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Anti-IL-8 antibody |
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| STJ97720 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Mouse monoclonal to IL-8. |
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 1730 8 SNAP-SEAL 8 OZ |
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| 1730-8 | CORNING | 100/pk | EUR 88.8 |
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Description: Disposable Plastic; Plastic Containers |
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 Human IL-8 |
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| 90258-A | BPS Bioscience | 5 µg | EUR 130 |
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Description: Recombinant human Interleukin-8 is a disulfide-linked monomer protein consisting of 78 amino acid residues, migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-8 mature chain was expressed in E. coli. |
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Human IL-8 |
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| 90258-B | BPS Bioscience | 25 µg | EUR 205 |
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Description: Recombinant human Interleukin-8 is a disulfide-linked monomer protein consisting of 78 amino acid residues, migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-8 mature chain was expressed in E. coli. |
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 mAb mouse anti-human IL-8 |
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| CT264 | U-CyTech | 0.5 mg | EUR 312 |
 IL-8 |
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| E5-00004 | EnoGene | 1mg | EUR 1152 |
 Anti-IL-8 Monoclonal Antibody |
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| A00423-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Mouse Monoclonal Antibody for IL-8 Antibody (CXCL8) detection. Tested with IHC in Human, Mouse, Rat. |
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 Individual Reaction Mix 8 |
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| G065-8 | ABM | 200 reactions | EUR 200.4 |
 Rabbit Polyclonal antibody Anti-CRBN |
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| Anti-CRBN | ImmunoStep | 50 µg | EUR 418.8 |
 Swine IL-6 Recombinant Protein |
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| R00102-8 | BosterBio | 5ug/vial | EUR 310.8 |
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Description: Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. Swine IL-6 Recombinant Protein is purified interleukin-6 produced in yeast. |
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 Swine IL-4 Recombinant Protein |
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| R00230-8 | BosterBio | 5ug/vial | EUR 310.8 |
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Description: IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. Swine IL-4 Recombinant Protein is purified interleukin-4 produced in yeast. |
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 Rabbit IL-2 Recombinant Protein |
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| R00387-8 | BosterBio | 5ug/vial | EUR 310.8 |
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Description: Interleukin-2 (IL-2) is a cytokine produced by T-helper cells in response to antigenic or mitogenic stimulation. It is required for T-cell proliferation and other activities crucial to the regulation of the immune response. Rabbit IL-2 Recombinant Protein is purified interleukin-2 produced in yeast. |
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 Swine IL-17A Recombinant Protein |
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| R00421-8 | BosterBio | 5ug/vial | EUR 310.8 |
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Description: IL-17A is a member of the IL-17 family of cytokines, whose members are involved in numerous immune regulatory functions. IL-17 induces the production of many other cytokines, chemokines, and prostaglandins. Swine IL-17A Recombinant Protein is purified interleukin-17A produced in yeast. |
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 Antibody) Human Interleukin-8 (IL-8) Antibody |
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| 13102-05011 | AssayPro | 150 ug | EUR 248.4 |
 Human IL-8 Antibody |
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| 33486-05111 | AssayPro | 150 ug | EUR 313.2 |
 Human IL-8 Protein |
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| abx060803-25ug | Abbexa | 25 ug | EUR 644.4 |
Human IL-8 Protein |
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| abx060804-25ug | Abbexa | 25 ug | EUR 644.4 |
 Recombinant Human IL-8 |
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| P0180 | FN Test | 100ug | EUR 626.83 |
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Description: Recombinant Human protein for IL-8 |
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 IL-8/CXCL8, Human |
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| HY-P7224 | MedChemExpress | 50ug | EUR 596.4 |
Recombinant Human IL-8 |
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| SJB05-01 | Amyotop | 25µg/vial | EUR 342 |
 Human THSD7a ELISA kit |
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| 8-E01A2155 | BlueGene |
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Description: A competitive ELISA for quantitative measurement of Human THSD7a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Ovine IL-1 beta Recombinant Protein |
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| R00101-8 | BosterBio | 5ug/vial | EUR 310.8 |
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Description: IL-1 beta (IL-1β) is a member of the interleukin 1 family of cytokines. The IL-1 beta cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. Ovine IL-1 beta Recombinant Protein is purified interleukin-1 beta cytokine produced in yeast. |
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 Sprint™ 8 Clinical Centrifuge with 8 x 15ml fixed angle rotor, 115V |
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| C5000-8 | Benchmark Scientific | 1 each | EUR 537.8 |
 antibody) Anti-bovine IL-8 (CXCL8) antibody |
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| STJ15100008 | St John's Laboratory | 250 µg | EUR 403.2 |
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Description: These monoclonal antibodies enable sensitive and specific detection of bovine IL-8 in ELISpot. |
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Anti-bovine IL-8 (CXCL8) antibody |
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| STJ15100009 | St John's Laboratory | 250 µg | EUR 403.2 |
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Description: This monoclonal antibody enables sensitive and specific detection of bovine IL-8 in ELISA. |
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 IL-8, Interleukin-8, monkey |
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| RC222-19 | Bio Basic | 5ug | EUR 125.26 |
 IL-8 Inhibitor |
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| 5-01378 | CHI Scientific | 4 x 5mg | Ask for price |
 IL-8 Antibody |
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| BF0238 | Affbiotech | 200ul | EUR 540 |
 CXCL8/IL-8 |
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| E21-C97 | EnoGene | 10ug | EUR 411.6 |
CXCL8/IL-8 |
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| GT15156 | Neuromics | 100 ug | EUR 631.2 |
IL-8 Inhibitor |
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| H-2268.0001 | Bachem | 1.0mg | EUR 200.4 |
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Description: Sum Formula: C45H66N18O7S; CAS# [138559-60-1] net |
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IL-8 Inhibitor |
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| H-2268.0005 | Bachem | 5.0mg | EUR 691.2 |
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Description: Sum Formula: C45H66N18O7S; CAS# [138559-60-1] net |
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IL-8 Inhibitor |
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| H-2268.0025 | Bachem | 25.0mg | EUR 2648.4 |
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Description: Sum Formula: C45H66N18O7S; CAS# [138559-60-1] net |
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) Bovine(IL-8) |
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| QY-E60063 | Qayee Biotechnology | 96T | EUR 511.2 |
IL-8 Antibody |
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| R30420 | NSJ Bioreagents | 100 ug | EUR 419 |
IL-8 Antibody |
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| R31932 | NSJ Bioreagents | 100 ug | EUR 419 |
IL-8 Antibody |
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| F51235-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 165 |
IL-8 Antibody |
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| F51235-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 379 |
IL-8 Antibody |
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| 5149-100 | Biovision | each | EUR 405.6 |
 CLIA Kit) Human Interleukin 8 (IL-8) CLIA Kit |
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| abx195916-96tests | Abbexa | 96 tests | EUR 990 |
 Human Interleukin 8,IL-8 ELISA KIT |
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| 201-12-0090 | SunredBio | 96 tests | EUR 528 |
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Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
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 Human Interleukin 8, IL-8 ELISA KIT |
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| CSB-E04641h-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
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Description: Quantitative sandwich ELISA kit for measuring Human Interleukin 8, IL-8 in samples from serum, cell culture supernates, saliva, urine, cerebrospinalfluid (CSF), tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
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Human Interleukin 8, IL-8 ELISA KIT |
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| 1-CSB-E04641h | Cusabio |
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Description: Quantitative sandwich ELISA kit for measuring Human Interleukin 8, IL-8 in samples from serum, cell culture supernates, saliva, urine, cerebrospinalfluid(CSF), tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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 ELISA Kit) Human IL-8(Interleukin 8) ELISA Kit |
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| EH0205 | FN Test | 96T | EUR 571.5 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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 ELISA Kit) Human Interleukin-8 (IL-8) ELISA Kit |
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| LF-EK60006 | Abfrontier | 1×96T | EUR 948 |
ELISA Kit) Human Interleukin 8(IL-8)ELISA Kit |
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| GA-E0129HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human Interleukin 8(IL-8)ELISA Kit |
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| GA-E0129HM-96T | GenAsia Biotech | 96T | EUR 559.2 |
Human Interleukin 8(IL-8)ELISA Kit |
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| QY-E04259 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Interleukin 8,IL-8 ELISA KIT |
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| YLA1210HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human Interleukin 8,IL-8 ELISA KIT |
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| YLA1210HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
 Human IL-8 ELISA kit |
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| CT212A | U-CyTech | 5-plate | EUR 554.4 |
 Human IL-8 ELISA Kit |
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| EHI0063 | Abclonal | 96Tests | EUR 625.2 |
 human IL-8 His tag |
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| E410A07-100 | EnoGene | 100μg | EUR 1152 |
 IL-8 ELISA KIT|Human |
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| EF003990 | Lifescience Market | 96 Tests | EUR 826.8 |
Human IL-8 ELISA kit |
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| LF-EK50165 | Abfrontier | 1×96T | EUR 777.6 |
) rec Monocyte IL-8 (human) |
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| H-9625.0005 | Bachem | 5.0µg | EUR 194.4 |
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Description: Sum Formula: C372H600N106O106S4; CAS# [142298-00-8] |
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rec Monocyte IL-8 (human) |
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| H-9625.0025 | Bachem | 25.0µg | EUR 457.2 |
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Description: Sum Formula: C372H600N106O106S4; CAS# [142298-00-8] |
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 IL-8/CXCL8, 72a.a., Human |
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| HY-P7380 | MedChemExpress | 50ug | EUR 596.4 |
) rec Endothelial IL-8 (human) |
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| H-3742.0010 | Bachem | 10.0µg | EUR 340.8 |
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Description: Sum Formula: C397H644N114O111S4; CAS# [142298-01-9] |
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rec Endothelial IL-8 (human) |
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| H-3742.0050 | Bachem | 50.0µg | EUR 1167.6 |
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Description: Sum Formula: C397H644N114O111S4; CAS# [142298-01-9] |
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 Recombinant Human IL-8 Protein |
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| RP00052 | Abclonal | 5 μg | EUR 178.8 |
 ELISA Kit) IL-8 (Human) ELISA Kit |
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| K4169-100 | Biovision | each | EUR 874.8 |
 ELISA Kit) Bovine Cytokines IL-8(Cytokines IL-8) ELISA Kit |
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| QY-E60102 | Qayee Biotechnology | 96T | EUR 511.2 |
 Pesticide Mix 8 Containing 15 Compounds in Acetonitrile |
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| SPXPR-8 | Scientific Laboratory Supplies | 1ML | EUR 249.66 |
 IL-8, CXCL8, Interleukin-8, canine |
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| RC242-19 | Bio Basic | 5ug | EUR 125.26 |
 IL-8, CXCL8, Interleukin-8, porcine |
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| RC282-19 | Bio Basic | 5ug | EUR 125.26 |
 ELISA) Horse Interleukin 8 (IL-8) ELISA |
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| QY-E120050 | Qayee Biotechnology | 96T | EUR 573.6 |
